Beta-Tomatine

CAS# 17406-46-1

Beta-Tomatine

Catalog No. BCX1122----Order now to get a substantial discount!

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Beta-Tomatine: 5mg Please Inquire In Stock
Beta-Tomatine: 10mg Please Inquire In Stock
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Quality Control of Beta-Tomatine

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Chemical structure

Beta-Tomatine

3D structure

Chemical Properties of Beta-Tomatine

Cas No. 17406-46-1 SDF Download SDF
PubChem ID 57343581.0 Appearance Powder
Formula C45H75NO17 M.Wt 902.09
Type of Compound Alkaloids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name (2S,3R,4S,5S,6R)-2-[(2S,3R,4S,5S,6R)-2-[(2R,3R,4R,5R,6R)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(1R,2S,4S,5'S,6S,7S,8R,9S,12S,13S,16S,18S)-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.02,9.04,8.013,18]icosane-6,2'-piperidine]-16-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
SMILES CC1CCC2(C(C3C(O2)CC4C3(CCC5C4CCC6C5(CCC(C6)OC7C(C(C(C(O7)CO)OC8C(C(C(C(O8)CO)O)O)OC9C(C(C(C(O9)CO)O)O)O)O)O)C)C)C)NC1
Standard InChIKey DMUPZSDWJVULSC-MMQVRIEESA-N
Standard InChI InChI=1S/C45H75NO17/c1-19-7-12-45(46-15-19)20(2)30-26(63-45)14-25-23-6-5-21-13-22(8-10-43(21,3)24(23)9-11-44(25,30)4)57-40-37(56)35(54)38(29(18-49)60-40)61-42-39(34(53)32(51)28(17-48)59-42)62-41-36(55)33(52)31(50)27(16-47)58-41/h19-42,46-56H,5-18H2,1-4H3/t19-,20-,21-,22-,23+,24-,25-,26-,27+,28+,29+,30-,31+,32+,33-,34-,35+,36+,37+,38-,39+,40+,41-,42-,43-,44-,45-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Beta-Tomatine

Tomato

Beta-Tomatine Dilution Calculator

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Beta-Tomatine Molarity Calculator

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Preparing Stock Solutions of Beta-Tomatine

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.1085 mL 5.5427 mL 11.0854 mL 22.1707 mL 27.7134 mL
5 mM 0.2217 mL 1.1085 mL 2.2171 mL 4.4341 mL 5.5427 mL
10 mM 0.1109 mL 0.5543 mL 1.1085 mL 2.2171 mL 2.7713 mL
50 mM 0.0222 mL 0.1109 mL 0.2217 mL 0.4434 mL 0.5543 mL
100 mM 0.0111 mL 0.0554 mL 0.1109 mL 0.2217 mL 0.2771 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Beta-Tomatine

Detoxification of alpha-tomatine by Cladosporium fulvum is required for full virulence on tomato.[Pubmed:23448507]

New Phytol. 2013 Jun;198(4):1203-1214.

. alpha-Tomatine is an antifungal glycoalkaloid that provides basal defense to tomato (Solanum lycopersicum). However, tomato pathogens overcome this basal defense barrier by the secretion of tomatinases that degrade alpha-tomatine into the less fungitoxic compounds Beta-Tomatine and tomatidine. Although pathogenic on tomato, it has been reported that the biotrophic fungus Cladosporium fulvum is unable to detoxify alpha-tomatine. . Here, we present a functional analysis of the glycosyl hydrolase (GH10), CfTom1, which is orthologous to fungal tomatinases. . We show that C. fulvum hydrolyzes alpha-tomatine into tomatidine in vitro and during the infection of tomato, which is fully attributed to the activity of CfTom1, as shown by the heterologous expression of this enzyme in tomato. Accordingly, ∆cftom1 mutants of C. fulvum are more sensitive to alpha-tomatine and are less virulent than the wild-type fungus on tomato. . Although alpha-tomatine is thought to be localized in the vacuole, we show that it is also present in the apoplast, where it is hydrolyzed by CfTom1 on infection. The accumulation of tomatidine during infection appears to be toxic to tomato cells and does not suppress defense responses, as suggested previously. Altogether, our results show that CfTom1 is responsible for the detoxification of alpha-tomatine by C. fulvum, and is required for full virulence of this fungus on tomato.

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