Chonglou Saponin VII

The roots of Paris polyphylla

Quantitative evaluation and discrimination of wild Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz from three regions of Yunnan Province using UHPLC-UV-MS and UV spectroscopy couple with partial least squares discriminant analysis.[Pubmed:27665609]

J Nat Med. 2017 Jan;71(1):148-157.

Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz (PPY) is used widely as an anthelmintic, antimicrobial, and anti-tumor agent. Multiplicate analytical methods have been employed to discriminate PPY from different regions, as well as to identify regions most beneficial to the growing of this species. In this study, a convenient and accurate method was established using ultra high performance liquid chromatography (UHPLC) for simultaneous determination of four steroid saponins (Pa, Pb, polyphyllin VI, and Chonglou Saponin VII). Partial least squares discriminant analysis (PLS-DA) according to UHPLC and UV spectroscopy was applied to analyze 30 samples of PPY from three regions of Yunnan Province in China, and identify significant peaks. The results indicated that the correlation coefficients (r (2)) of all calibration curves were above 0.999, and the inter- and intra-day relative standard deviations (RSD) of retention time and peak areas of common peaks were below 1.78 % and 3.40 %, respectively, with recovery rates of 99.6-103.4 % with RSD

[Effects of Paris polyphylla saponin VII plus silica nano composite on ovarian cancer drug resistance in vitro].[Pubmed:26712407]

Zhonghua Yi Xue Za Zhi. 2015 Jun 16;95(23):1859-61.

OBJECTIVE: To explore the drug resistance of Chonglou Saponin VII plus silica nano complex for ovarian cancer. METHODS: The method of thiazolyl blue tetrazolium blue (MTT) was employed for determining the proliferation inhibition rate of Chonglou Saponin VII plus silica nano composite for SKOV3/DDP drug-resistant human ovarian cancer cell. Flow cytometry and Hoechst 33342 staining were used for detecting apoptosis rate and cell cycle phase distribution. And the changes of apoptosis-related protein were examined by immunoblot assay. RESULTS: There were obvious inhibitory effects of Chonglou Saponin VII on cell proliferation. The inhibition rate increased with rising drug concentration with an obvious dose-effect relationship (P < 0.05). At the same time, the cell proliferation inhibition rate significantly improved at 48 h versus 24 h (P < 0.05). Flow cytometry and Hoechst 33342 staining showed that, after a 24 h treatment of SKOV3/DDP cells with 50 and 100 micromol/L Chonglou Saponin VII, early apoptotic cell ratio significantly increased compared with control group. CONCLUSION: The composite of Paris polyphylla saponin VII and silica nano has obvious proliferation inhibitory effects on SKOV3/DDP cells. And there is a significant concentration dependence and it also has some correlation with time. The above composite can increased the proportion of early apoptotic cell ratio.

Paris saponin VII (Chonglou Saponin VII) is a steroidal saponin isolated from the roots and rhizomes of Trillium tschonoskii Maxim. Paris saponin VII-induced apoptosis in K562/ADR cells is associated with Akt/MAPK and the inhibition of P-gp. Paris saponin VII attenuates mitochondrial membrane potential, increases the expression of apoptosis-related proteins, such as Bax and cytochrome c, and decreases the protein expression levels of Bcl-2, caspase-9, caspase-3, PARP-1, and p-Akt. Paris saponin VII induces a robust autophagy in K562/ADR cells and provides a biochemical basis in the treatment of leukemia.

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