Ercalcitriol

Active metabolite of vitamin D2 CAS# 60133-18-8

Ercalcitriol

Catalog No. BCC1556----Order now to get a substantial discount!

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Quality Control of Ercalcitriol

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Chemical structure

Ercalcitriol

3D structure

Chemical Properties of Ercalcitriol

Cas No. 60133-18-8 SDF Download SDF
PubChem ID 9547243 Appearance Powder
Formula C28H44O3 M.Wt 428.66
Type of Compound N/A Storage Desiccate at -20°C
Synonyms 1α,25-Dihydroxyvitamin D2
Solubility DMSO : ≥ 50 mg/mL (116.65 mM)
*"≥" means soluble, but saturation unknown.
Chemical Name (1R,3S,5Z)-5-[(2E)-2-[(1R,3aS,7aR)-1-[(E,2R,5S)-6-hydroxy-5,6-dimethylhept-3-en-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol
SMILES CC(C=CC(C)C(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CC(C3=C)O)O)C
Standard InChIKey ZGLHBRQAEXKACO-XJRQOBMKSA-N
Standard InChI InChI=1S/C28H44O3/c1-18(9-10-19(2)27(4,5)31)24-13-14-25-21(8-7-15-28(24,25)6)11-12-22-16-23(29)17-26(30)20(22)3/h9-12,18-19,23-26,29-31H,3,7-8,13-17H2,1-2,4-6H3/b10-9+,21-11+,22-12-/t18-,19+,23-,24-,25+,26+,28-/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of Ercalcitriol

DescriptionActive metabolite of vitamin D2. Exhibits equipotent antirachitic activity in rats as calcitriol.

Ercalcitriol Dilution Calculator

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Ercalcitriol Molarity Calculator

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Preparing Stock Solutions of Ercalcitriol

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.3329 mL 11.6643 mL 23.3285 mL 46.657 mL 58.3213 mL
5 mM 0.4666 mL 2.3329 mL 4.6657 mL 9.3314 mL 11.6643 mL
10 mM 0.2333 mL 1.1664 mL 2.3329 mL 4.6657 mL 5.8321 mL
50 mM 0.0467 mL 0.2333 mL 0.4666 mL 0.9331 mL 1.1664 mL
100 mM 0.0233 mL 0.1166 mL 0.2333 mL 0.4666 mL 0.5832 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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Background on Ercalcitriol

Ercalcitriol (1α,25-Dihydroxy Vitamin D2) is a active metabolite of vitamin D2. Ercalcitriol (1α,25-Dihydroxy Vitamin D2) exhibits equipotent antirachitic activity in rats as calcitriol. Ercalcitriol(1α,25-Dihydroxy Vitamin D2) is a VDR-like Receptors

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References on Ercalcitriol

A sensitive radioimmunoassay using a monoclonal antibody that is equipotent for ercalcitriol and calcitriol (1,25-dihydroxy vitamin D2 and D3).[Pubmed:2253400]

Clin Chim Acta. 1990 Oct 15;190(3):199-209.

A monoclonal antibody has been used in a sensitive radioimmunoassay that measures 1,25-dihydroxyvitamin D2 (Ercalcitriol) and 1,25-dihydroxyvitamin D3 (calcitriol) with equal potency. This important characteristic has not been reported for any other radioimmunoassay for 1,25-dihydroxyvitamin D. The two forms can be assayed in human serum together or individually after HPLC separation. Sample preparation entails acetonitrile extraction followed by C18-Sep-pak chromatography and HPLC. The assay measures 98% of added analyte, and achieves inter- and intra-assay coefficients of variation of 10.7% at 34 pg/ml and 7.8% at 81 pg/ml respectively. The limit of detection is 1.25 pg/tube and 50% displacement of bound ligand is achieved at 14 pg/tube. The reference interval is 20-50 pg/ml, mean 35. The correlation between results from the monoclonal radioimmunoassay and an established polyclonal antibody method was r = 0.98, slope 0.99. The assay has particular application in patients treated with vitamin D2 since 1,25-dihydroxyvitamin D2 can now be measured accurately in the presence of 1,25-dihydroxyvitamin D3.

Description

Ercalcitriol (1α,25-Dihydroxy Vitamin D2) is an active metabolite of vitamin D2.

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