Esculentoside E

CAS# 65649-36-7

Esculentoside E

2D Structure

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Esculentoside E

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Chemical Properties of Esculentoside E

Cas No. 65649-36-7 SDF Download SDF
PubChem ID 128258 Appearance Powder
Formula C35H54O11 M.Wt 650.80
Type of Compound Triterpenoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name (2R,4aR,6aR,6aS,6bR,9R,10R,11S,12aR,14bR)-11-hydroxy-9-(hydroxymethyl)-2,6a,6b,9,12a-pentamethyl-10-[(2S,3R,4S,5R)-3,4,5-trihydroxyoxan-2-yl]oxy-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-2,4a-dicarboxylic acid
SMILES CC1(CCC2(CCC3(C(=CCC4C3(CCC5C4(CC(C(C5(C)CO)OC6C(C(C(CO6)O)O)O)O)C)C)C2C1)C)C(=O)O)C(=O)O
Standard InChIKey UERRXLUEVHKNBY-JDNGKTEJSA-N
Standard InChI InChI=1S/C35H54O11/c1-30(28(41)42)10-12-35(29(43)44)13-11-33(4)18(19(35)14-30)6-7-23-31(2)15-20(37)26(46-27-25(40)24(39)21(38)16-45-27)32(3,17-36)22(31)8-9-34(23,33)5/h6,19-27,36-40H,7-17H2,1-5H3,(H,41,42)(H,43,44)/t19-,20+,21-,22?,23-,24+,25-,26+,27+,30-,31+,32+,33-,34-,35+/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Esculentoside E

The roots of Phytolacca acinosa Roxb

Protocol of Esculentoside E

Structure Identification
Acta pharmaceutica sinica, 1984.

STUDIES ON THE ACTIVE PRINCIPLES OF THE CHINESE DRUG "SHANG LU" (PHYTOLACCA ESCULENTA VAN HO UTTE)Ⅱ. THE ISOLATION AND STRUCTURE OF ESCULENTOSIDE E AND F.[Reference: WebLink]

In a previous paper we reported the isolation of esculentoside A, B, C and D from Phytolacca eseulenta Van Houtte.In pharmacological experiments the total saponins and esculentoside A exerted considerable enhancement of phagocytic function of leucocytes and promoted DNA synthesis in mice. .
METHODS AND RESULTS:
In further search for active principles, four kinds of crystals (Ⅰ), (Ⅱ), (Ⅲ) and (Ⅳ) were isolated from the 0.01 M pH 7.3 phosphate buffer extract of the plant by silica gel column chromatography. On the basis of chemical properties and spectral data (NMR, ~(13)CNMR, UV, IR, Ms spectra), crystal (Ⅰ) was shown to be 2-hydroxyl esculentic acid, i.e. jaligonic acid which has been considered to have good antiinflammatory action; crystal (Ⅱ) was found to be 3-O-β-D-xylopyranosyl-2-hydroxylesculentic acid and was named as esculentosid E which is identical to phytolaccoside G from Phytolacca americana. Crystal (Ⅲ) is 3-O(β-D-glucopyranosyl-β-D-xylopyranosyl)_(1→4)-2-hydroxyl esculentic acid named as esculentoside F.
CONCLUSIONS:
Esculentoside E was first obtained from this plant and esculentoside F so far has not been reported in literature. Because of the scanty sample of crystal (Ⅳ), its structure is still under investigation.

Esculentoside E Dilution Calculator

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Esculentoside E Molarity Calculator

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Preparing Stock Solutions of Esculentoside E

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.5366 mL 7.6829 mL 15.3657 mL 30.7314 mL 38.4143 mL
5 mM 0.3073 mL 1.5366 mL 3.0731 mL 6.1463 mL 7.6829 mL
10 mM 0.1537 mL 0.7683 mL 1.5366 mL 3.0731 mL 3.8414 mL
50 mM 0.0307 mL 0.1537 mL 0.3073 mL 0.6146 mL 0.7683 mL
100 mM 0.0154 mL 0.0768 mL 0.1537 mL 0.3073 mL 0.3841 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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