Fumaric acid

CAS# 110-17-8

Fumaric acid

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Quality Control of Fumaric acid

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Chemical structure

Fumaric acid

3D structure

Chemical Properties of Fumaric acid

Cas No. 110-17-8 SDF Download SDF
PubChem ID 444972 Appearance White powder
Formula C4H4O4 M.Wt 116.1
Type of Compound Miscellaneous Storage Desiccate at -20°C
Synonyms Allomaleic acid; trans-2-Butenedioic acid; Lichenic acid
Solubility Soluble in ethanol; slightly soluble in acetone and water
Chemical Name (E)-but-2-enedioic acid
SMILES C(=CC(=O)O)C(=O)O
Standard InChIKey VZCYOOQTPOCHFL-OWOJBTEDSA-N
Standard InChI InChI=1S/C4H4O4/c5-3(6)1-2-4(7)8/h1-2H,(H,5,6)(H,7,8)/b2-1+
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Fumaric acid

The herbs of Fumaria officinalis

Biological Activity of Fumaric acid

DescriptionFumaric acid is an intermediate in the citric acid cycle used by cells to produce energy in the form of adenosine triphosphate (ATP) from food; also a product of the urea cycle. Fumaric acid is used in systemic and topical treatment of psoriasis. Fumaric acid attenuates the eotaxin-1 expression in TNF-α-stimulated fibroblasts by suppressing p38 MAPK-dependent NF-κB signaling.
TargetsTNF-α | NF-kB | p38MAPK
In vitro

Metabolic engineering of Escherichia coli for the production of fumaric acid.[Pubmed: 23436277]

Biotechnol Bioeng. 2013 Jul;110(7):2025-34.


METHODS AND RESULTS:
In this study, Escherichia coli was metabolically engineered for the production of Fumaric acid under aerobic condition. For the aerobic production of Fumaric acid, the iclR gene was deleted to redirect the carbon flux through the glyoxylate shunt. In addition, the fumA, fumB, and fumC genes were also deleted to enhance Fumaric acid formation. The resulting strain was able to produce 1.45 g/L of Fumaric acid from 15 g/L of glucose in flask culture. Based on in silico flux response analysis, this base strain was further engineered by plasmid-based overexpression of the native ppc gene, encoding phosphoenolpyruvate carboxylase (PPC), from the strong tac promoter, which resulted in the production of 4.09 g/L of Fumaric acid. Additionally, the arcA and ptsG genes were deleted to reinforce the oxidative TCA cycle flux, and the aspA gene was deleted to block the conversion of Fumaric acid into L-aspartic acid. Since it is desirable to avoid the use of inducer, the lacI gene was also deleted. To increase glucose uptake rate and Fumaric acid productivity, the native promoter of the galP gene was replaced with the strong trc promoter. Fed-batch culture of the final strain CWF812 allowed production of 28.2 g/L Fumaric acid in 63 h with the overall yield and productivity of 0.389 g Fumaric acid/g glucose and 0.448 g/L/h, respectively.
CONCLUSIONS:
This study demonstrates the possibility for the efficient production of Fumaric acid by metabolically engineered E. coli.

Protocol of Fumaric acid

Kinase Assay

Fumaric acid attenuates the eotaxin-1 expression in TNF-α-stimulated fibroblasts by suppressing p38 MAPK-dependent NF-κB signaling.[Pubmed: 23707484]

Food Chem Toxicol. 2013 Aug;58:423-31.

In this study, we investigated the effects of Fumaric acid on eotaxin-1 expression in a mouse fibroblast cell line.
METHODS AND RESULTS:
We found that Fumaric acid significantly inhibited tumor necrosis factor-α (TNF-α-induced eotaxin-1 expression. This Fumaric acid effect was mediated through the inhibition of p38 mitogen-activated protein kinase (MAPK)-dependent nuclear factor (NF)-κB signaling. We also found that Fumaric acid operates downstream of MEKK3 during TNF-α-induced NF-κB signaling, which upregulated eotaxin-1 expression. In addition, Fumaric acid attenuated expression of CC-chemokine receptor 3 (CCR3), an eotaxin-1 receptor, and adhesion molecules that play important roles in eosinophil binding to induce allergic inflammation.
CONCLUSIONS:
Taken together, these findings indicate that inhibiting TNF-α-induced eotaxin-1 expression by Fumaric acid occurs primarily through suppression of NF-κB signaling, which is mediated by inhibiting p38 MAPK and suggest that Fumaric acid may be used as a complementary treatment option for eotaxin-1-mediated diseases.

Cell Research

Fumaric acid production by Torulopsis glabrata: engineering the urea cycle and the purine nucleotide cycle.[Pubmed: 25060134]

Biotechnol Bioeng. 2015 Jan;112(1):156-67.

A multi-vitamin auxotrophic Torulopsis glabrata strain, a pyruvate producer, was further engineered to produce Fumaric acid.
METHODS AND RESULTS:
Using the genome-scale metabolic model iNX804 of T. glabrata, four Fumaric acid biosynthetic pathways, involving the four cytosolic enzymes, argininosuccinate lyase (ASL), adenylosuccinate lyase (ADSL), fumarylacetoacetase (FAA), and fumarase (FUM1), were found. Athough single overexpression of each of the four enzymes in the cytosol improved Fumaric acid production, the highest Fumaric acid titer (5.62 g L(-1) ) was obtained with strain T.G-ASL(H) -ADSL(L) by controlling the strength of ASL at a high level and ADSL at a low level. In order to further improve the production of Fumaric acid, the SpMAE1 gene encoding the C4 -dicarboxylic acids transporter was overexpressed in strain T.G-ASL(H) -ADSL(L) -SpMAE1 and the final Fumaric acid titer increased to 8.83 g L(-1) .
CONCLUSIONS:
This study provides a novel strategy for Fumaric acid biosynthesis by utilizing the urea cycle and the purine nucleotide cycle to enhance the bridge between carbon metabolism and nitrogen metabolism.

Fumaric acid Dilution Calculator

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Preparing Stock Solutions of Fumaric acid

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 8.6133 mL 43.0663 mL 86.1326 mL 172.2653 mL 215.3316 mL
5 mM 1.7227 mL 8.6133 mL 17.2265 mL 34.4531 mL 43.0663 mL
10 mM 0.8613 mL 4.3066 mL 8.6133 mL 17.2265 mL 21.5332 mL
50 mM 0.1723 mL 0.8613 mL 1.7227 mL 3.4453 mL 4.3066 mL
100 mM 0.0861 mL 0.4307 mL 0.8613 mL 1.7227 mL 2.1533 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Fumaric acid

Metabolic engineering of Escherichia coli for the production of fumaric acid.[Pubmed:23436277]

Biotechnol Bioeng. 2013 Jul;110(7):2025-34.

Fumaric acid is a naturally occurring organic acid that is an intermediate of the tricarboxylic acid cycle. Fungal species belonging to Rhizopus have traditionally been employed for the production of Fumaric acid. In this study, Escherichia coli was metabolically engineered for the production of Fumaric acid under aerobic condition. For the aerobic production of Fumaric acid, the iclR gene was deleted to redirect the carbon flux through the glyoxylate shunt. In addition, the fumA, fumB, and fumC genes were also deleted to enhance Fumaric acid formation. The resulting strain was able to produce 1.45 g/L of Fumaric acid from 15 g/L of glucose in flask culture. Based on in silico flux response analysis, this base strain was further engineered by plasmid-based overexpression of the native ppc gene, encoding phosphoenolpyruvate carboxylase (PPC), from the strong tac promoter, which resulted in the production of 4.09 g/L of Fumaric acid. Additionally, the arcA and ptsG genes were deleted to reinforce the oxidative TCA cycle flux, and the aspA gene was deleted to block the conversion of Fumaric acid into L-aspartic acid. Since it is desirable to avoid the use of inducer, the lacI gene was also deleted. To increase glucose uptake rate and Fumaric acid productivity, the native promoter of the galP gene was replaced with the strong trc promoter. Fed-batch culture of the final strain CWF812 allowed production of 28.2 g/L Fumaric acid in 63 h with the overall yield and productivity of 0.389 g Fumaric acid/g glucose and 0.448 g/L/h, respectively. This study demonstrates the possibility for the efficient production of Fumaric acid by metabolically engineered E. coli.

Fumaric acid attenuates the eotaxin-1 expression in TNF-alpha-stimulated fibroblasts by suppressing p38 MAPK-dependent NF-kappaB signaling.[Pubmed:23707484]

Food Chem Toxicol. 2013 Aug;58:423-31.

Eotaxin-1 is a potent chemoattractant for eosinophils and a critical mediator during the development of eosinophilic inflammation. Fumaric acid is an intermediate product of the citric acid cycle, which is source of intracellular energy. Although Fumaric acid ameliorates psoriasis and multiple sclerosis, its involvement in eotaxin-1-mediated effects has not been assessed. In this study, we investigated the effects of Fumaric acid on eotaxin-1 expression in a mouse fibroblast cell line. We found that Fumaric acid significantly inhibited tumor necrosis factor-alpha (TNF-alpha-induced eotaxin-1 expression. This Fumaric acid effect was mediated through the inhibition of p38 mitogen-activated protein kinase (MAPK)-dependent nuclear factor (NF)-kappaB signaling. We also found that Fumaric acid operates downstream of MEKK3 during TNF-alpha-induced NF-kappaB signaling, which upregulated eotaxin-1 expression. In addition, Fumaric acid attenuated expression of CC-chemokine receptor 3 (CCR3), an eotaxin-1 receptor, and adhesion molecules that play important roles in eosinophil binding to induce allergic inflammation. Taken together, these findings indicate that inhibiting TNF-alpha-induced eotaxin-1 expression by Fumaric acid occurs primarily through suppression of NF-kappaB signaling, which is mediated by inhibiting p38 MAPK and suggest that Fumaric acid may be used as a complementary treatment option for eotaxin-1-mediated diseases.

Fumaric acid production by Torulopsis glabrata: engineering the urea cycle and the purine nucleotide cycle.[Pubmed:25060134]

Biotechnol Bioeng. 2015 Jan;112(1):156-67.

A multi-vitamin auxotrophic Torulopsis glabrata strain, a pyruvate producer, was further engineered to produce Fumaric acid. Using the genome-scale metabolic model iNX804 of T. glabrata, four Fumaric acid biosynthetic pathways, involving the four cytosolic enzymes, argininosuccinate lyase (ASL), adenylosuccinate lyase (ADSL), fumarylacetoacetase (FAA), and fumarase (FUM1), were found. Athough single overexpression of each of the four enzymes in the cytosol improved Fumaric acid production, the highest Fumaric acid titer (5.62 g L(-1) ) was obtained with strain T.G-ASL(H) -ADSL(L) by controlling the strength of ASL at a high level and ADSL at a low level. In order to further improve the production of Fumaric acid, the SpMAE1 gene encoding the C4 -dicarboxylic acids transporter was overexpressed in strain T.G-ASL(H) -ADSL(L) -SpMAE1 and the final Fumaric acid titer increased to 8.83 g L(-1) . This study provides a novel strategy for Fumaric acid biosynthesis by utilizing the urea cycle and the purine nucleotide cycle to enhance the bridge between carbon metabolism and nitrogen metabolism.

Description

Fumaric acid, associated with fumarase deficiency, is identified as an oncometabolite or an endogenous, cancer causing metabolite.

Keywords:

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