Succinic acid

CAS# 110-15-6

Succinic acid

2D Structure

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Quality Control of Succinic acid

3D structure

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Succinic acid

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Chemical Properties of Succinic acid

Cas No. 110-15-6 SDF Download SDF
PubChem ID 1110 Appearance Powder
Formula C4H6O4 M.Wt 118.1
Type of Compound Miscellaneous Storage Desiccate at -20°C
Solubility DMSO : 100 mg/mL (846.81 mM; Need ultrasonic)
H2O : 30 mg/mL (254.04 mM; Need ultrasonic)
Chemical Name butanedioic acid
SMILES C(CC(=O)O)C(=O)O
Standard InChIKey KDYFGRWQOYBRFD-UHFFFAOYSA-N
Standard InChI InChI=1S/C4H6O4/c5-3(6)1-2-4(7)8/h1-2H2,(H,5,6)(H,7,8)
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Succinic acid

The rhizome of Gastrodia elata

Biological Activity of Succinic acid

DescriptionSuccinic acid is a crystalline organic acid which occurs in living tissue as an intermediate in glucose metabolism.

Protocol of Succinic acid

Structure Identification
Bioresour Technol. 2015 Feb 26;185C:56-61.

Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli.[Pubmed: 25747879]

In this study, microbial production of Succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of Succinic acid on mannitol (1.39±0.01mol/mol) than glucose (1.01±0.05mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31±0.32g/L) and mannitol (10.12±0.17g/L), which was used as the substrate for Succinic acid fermentation with the recombinant BS002. A final 17.44±0.54g/L Succinic acid was obtained from the hydrolysate after 72h dual-phase fermentation. The yield was as high as 1.24±0.08mol/mol total sugar, which reached 73% of the maximum theoretical yield.

Succinic acid Dilution Calculator

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Succinic acid Molarity Calculator

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Preparing Stock Solutions of Succinic acid

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 8.4674 mL 42.337 mL 84.674 mL 169.348 mL 211.685 mL
5 mM 1.6935 mL 8.4674 mL 16.9348 mL 33.8696 mL 42.337 mL
10 mM 0.8467 mL 4.2337 mL 8.4674 mL 16.9348 mL 21.1685 mL
50 mM 0.1693 mL 0.8467 mL 1.6935 mL 3.387 mL 4.2337 mL
100 mM 0.0847 mL 0.4234 mL 0.8467 mL 1.6935 mL 2.1169 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Succinic acid

Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli.[Pubmed:25747879]

Bioresour Technol. 2015 Jun;185:56-61.

In this study, microbial production of Succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of Succinic acid on mannitol (1.39+/-0.01mol/mol) than glucose (1.01+/-0.05mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31+/-0.32g/L) and mannitol (10.12+/-0.17g/L), which was used as the substrate for Succinic acid fermentation with the recombinant BS002. A final 17.44+/-0.54g/L Succinic acid was obtained from the hydrolysate after 72h dual-phase fermentation. The yield was as high as 1.24+/-0.08mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production.

Capture of carbon dioxide from ethanol fermentation by liquid absorption for use in biological production of succinic acid.[Pubmed:25448631]

Appl Biochem Biotechnol. 2015 Feb;175(4):2104-13.

Previously, it was shown that the gas produced in an ethanol fermentor using either corn or barley as feedstock could be sparged directly into an adjacent fermentor as a feedstock for Succinic acid fermentation using Escherichia coli AFP184. In the present investigation, it was demonstrated that the CO2 produced in a corn ethanol fermentor could be absorbed in a base solution and the resultant carbonate solution used both for pH control and supply of the CO2 requirement in Succinic acid fermentation. Thus, the CO2 produced in a 5-L corn mash containing 30 wt% total solids was absorbed in a packed column containing 2 L of either 5 M NaOH, 5 M KOH, or 15 wt% NH4OH, and the resultant carbonate solutions were used for pH control in a Succinic acid fermentor. The results obtained indicated no significant differences between Succinic acid production in these experiments and when 2.5 M solutions of Na2CO3, K2CO3, and (NH4)2CO3 from commercial sources were used. In a commercial setting, the demonstrated capture of CO2 in liquid form will allow transportation of the carbonate solutions to locations not in the immediate vicinity of the ethanol plant, and excess carbonate salts can also be recovered as value-added products.

Description

Succinic acid is an intermediate product of the tricarboxylic acid cycle, as well as one of fermentation products of anaerobic metabolism.

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