Ionomycin calcium saltionophore CAS# 56092-82-1 |
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Quality Control & MSDS
3D structure
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| Cas No. | 56092-82-1 | SDF | Download SDF |
| PubChem ID | 71308788 | Appearance | Powder |
| Formula | C41H70CaO9 | M.Wt | 747.08 |
| Type of Compound | N/A | Storage | Desiccate at -20°C |
| Solubility | DMSO : ≥ 10 mg/mL (13.39 mM) *"≥" means soluble, but saturation unknown. | ||
| Chemical Name | (4R,6S,8S,10Z,12R,14R,16E,18R,19R,2 | ||
| SMILES | CC(CCC(=O)[O-])CC(C)CC(C)C(=O)C=C(C(C)CC(C)CC=CC(C)C(C(C)C(CC1CCC(O1)(C)C2CCC(O2)(C)C(C)O)O)O)[O-].[Ca+2] | ||
| Standard InChIKey | WKRWUYKLUMMAKG-WYGBAUISSA-L | ||
| Standard InChI | InChI=1S/C41H71O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42,45,48H,12,14-23H2,1-10H3,(H,46,47);/q-1;+2/p-1/b13-11+;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
| Description | Calcium ionophore; more specific than A23187. |
Ionomycin calcium salt Dilution Calculator
Ionomycin calcium salt Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 1.3385 mL | 6.6927 mL | 13.3854 mL | 26.7709 mL | 33.4636 mL |
| 5 mM | 0.2677 mL | 1.3385 mL | 2.6771 mL | 5.3542 mL | 6.6927 mL |
| 10 mM | 0.1339 mL | 0.6693 mL | 1.3385 mL | 2.6771 mL | 3.3464 mL |
| 50 mM | 0.0268 mL | 0.1339 mL | 0.2677 mL | 0.5354 mL | 0.6693 mL |
| 100 mM | 0.0134 mL | 0.0669 mL | 0.1339 mL | 0.2677 mL | 0.3346 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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Ionomycin calcium salt is a calcium ionophore [1].
Ca2+ ionophore acts as a Ca2+ ionophore, allowing Ca2+ to cross cell membranes.
Ionomycin calcium salt is a calcium ionophore. In cultured chicken pectoralis muscle, ionomycin (10-6-10-5 M) selectively increased the incorporation of methionine into two proteins. Ionomycin increased influx of extracellular Ca2+ and increased Ca2+ concentration in the cytoplasm, which then regulated the synthesis of muscle proteins [2]. In rat parotid gland, ionomycin increased 86Rb efflux, 22Na uptake and 3H-protein secretion, which were dependent on increasing cytosolic Ca2+. In parotid cells, ionomycin released the receptor-regulated cellular Ca2+ pool [3]. In human bladder cancer cells HT1376, ionomycin significantly inhibited cell growth in a time- and dose-dependent way and induced apoptotic DNA degradation. Also, ionomycin reduced the ratios of Bcl-2 to Bax mRNA and protein [4].
In athymic nude mice bearing HT1376 tumors, ionomycin significantly inhibited tumor growth and reduced the tumorigenicity [4].
References:
[1]. Liu C, Hermann TE. Characterization of ionomycin as a calcium ionophore. J Biol Chem, 1978, 253(17): 5892-5894.
[2]. Roufa D, Wu FS, Martonosi AN. The effect of Ca2+ ionophores upon the synthesis of proteins in cultured skeletal muscle. Biochim Biophys Acta, 1981, 674(2): 225-237.
[3]. Poggioli J, Leslie BA, McKinney JS, et al. Actions of ionomycin in rat parotid gland. J Pharmacol Exp Ther, 1982, 221(1): 247-253.
[4]. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. J Urol, 1999, 162(3 Pt 1): 916-921.
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Effects of chemical combinations on the parthenogenetic activation of mouse oocytes.[Pubmed:23737865]
Exp Ther Med. 2013 May;5(5):1281-1288.
The aim of this study was to identify an optimal method for the parthenogenetic activation of mouse oocytes. Ethanol (EH), strontium chloride (SrCl2) and Ionomycin calcium salt were each combined with cytochalasin B to induce the parthenogenetic activation of CD-1((R)) mouse oocytes. Among the EH combination groups, the blastocyst formation and hatching rates of the group that was activated with EH and CB for 5 min were significantly higher compared with those of the groups that were activated for 7 and 10 min (P<0.05). Among the SrCl2 combination groups, the blastocyst formation and hatching rates of the group that was activated with SrCl2 and CB for 30 min were significantly higher compared with those of the groups that were activated for 1 and 2 h (P<0.05). Among the Ionomycin calcium salt combination groups, the blastocyst formation and hatching rates of the group that was activated with ionomycin and CB for 3 min were higher compared with those of the groups that were activated for 5 and 7 min (P<0.05). Compared with the other two combinations, the experimental indicators of the EH combination groups were notably superior (P<0.05). For combined activation, simultaneous activation with two substances was significantly more effective than successive activation (P<0.05). For combined activation with EH and cytochalasin B in mouse oocytes, 5 min of parthenogenetic activation had significant advantages with regard to cleavage, blastocyst formation and blastocyst hatching rates. In addition, the activation rate of combined activation was higher than that of single activators. For combined activation, the simultaneous application of two activators has a superior effect.
Ionomycin causes activation of p38 and p42/44 mitogen-activated protein kinases in human neutrophils.[Pubmed:11401859]
Am J Physiol Cell Physiol. 2001 Jul;281(1):C350-60.
Many receptor-linked agents that prime or activate the NADPH oxidase in polymorphonuclear neutrophils (PMNs) elicit changes in cytosolic Ca2+ concentration and activate mitogen-activated protein (MAP) kinases. To investigate the role of Ca2+ in the activation of p38 and p42/44 MAP kinases, we examined the effects of the Ca2+-selective ionophore ionomycin on priming and activation of the PMN oxidase. Ionomycin caused a rapid rise in cytosolic Ca2+ that was due to both a release of cytosolic Ca2+ stores and Ca2+ influx. Ionomycin also activated (2 microM) and primed (20-200 nM) the PMN oxidase. Dual phosphorylation of p38 MAP kinase and phosphorylation of its substrate activating transcription factor-2 were detected at ionomycin concentrations that prime or activate the PMN oxidase, while dual phosphorylation of p42/44 MAP kinase and phosphorylation of its substrate Elk-1 were elicited at 0.2-2 microM. SB-203580, a p38 MAP kinase antagonist, inhibited ionomycin-induced activation of the oxidase (68 +/- 8%, P < 0.05) and tyrosine phosphorylation of 105- and 72-kDa proteins; conversely, PD-98059, an inhibitor of MAP/extracellular signal-related kinase 1, had no effect. Treatment of PMNs with thapsigargin resulted in priming of the oxidase and activation of p38 MAP kinase. Chelation of cytosolic but not extracellular Ca2+ completely inhibited ionomycin activation of p38 MAP kinase, whereas chelation of extracellular Ca2+ abrogated activation of p42/44 MAP kinase. These results demonstrate the importance of changes in cytosolic Ca2+ for MAP kinase activation in PMNs.
Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria.[Pubmed:6766939]
J Biol Chem. 1980 Apr 10;255(7):2735-9.
Based on the effects of ionomycin upon mitochondrial respiration, ionomycin was shown to be an effective ionophore for Ca2+ in rat liver mitochondria. The ionomycin-induced efflux of Ca2+ across the inner membrane was more sensitive to loading the mitochondria with Ca2+ than was efflux catalyzed by A23187. At saturating concentrations of Ca2+, the turnover number for ionomycin was 3- to 5-fold greater than that of A23187. Ionomycin catalyzed the efflux of mitochondrial Mg2+ at rates comparable to those observed with A23187. Ionomycin also mediated an efflux of K+ provided that the mitochondria were depleted of their endogenous divalent metal ions. The apparent turnover numbers for K+ efflux suggest that ionomycin is more specific for divalent metal ions than A23187.
Characterization of ionomycin as a calcium ionophore.[Pubmed:28319]
J Biol Chem. 1978 Sep 10;253(17):5892-4.
The ionophorous properties of a new antibiotic, ionomycin, have been studied. It was found that the antibiotic is capable of extracting calcium ion from the bulk of an aqueous phase into an organic phase. The antibiotic also acts as a mobile ion carrier to transport the cation across a solvent barrier. The divalent cation selectivity order for ionomycin as determined by ion competition experiments was found to be: Ca greater than Mg greater than Sr = Ba, where the binding of strontium and barium by the antibiotic is insignificant. The antibiotic also binds La3+ to some extent, but its complexation with monovalent alkali metal ions is negligible. Measurement of the binding of ionomycin with Ca2+ indicates that ionomycin complexes and transports calcium ion in a one to one stoichiometry.
