Methylgomisin O

Anti-inflammatory effect and mechanism of methylgomisin O.[Reference: WebLink]

Chinese journal of veterinary science, 2011, 31(9):1309-1308.

Methylgomisin O was a new dibenzocyclooctadiene lignan isolated from the fruits of Schisandra sphenanthera.
METHODS AND RESULTS:
In this paper,we investigated in vitro[LPS-induced murine RAW264.7 macrophages],the effect of Methylgomisin O on the generation of cytokines involved in the inflammatory process,such as TNF-α,IL-1β,IL-6 and IL-10.We further investigated the effect of Methylgomisin O on the LPS-induced activation of NF-κB and MAPK pathway.The result was that 0.5,2.5 and 12.5 mg/L of Methylgomisin O significantly decreased the production of TNF-α and IL-6,but didn't have significant effect on IL-1β and IL-10.Signal transduction studies showed that Methylgomisin O significantly inhibited p65-NF-κB and MAPK in a dose-dependence manner.
CONCLUSIONS:
Accordingly,we concluded that Methylgomisin O-induced attenuation of inflammatory cytokine production upon exposure to LPS was at least partially mediated by the suppression of NF-κB and MAPK pathway.

The Antiproliferative Effects of Compounds Isolated from Schisandra chinensis.[Reference: WebLink]

Korean journal of food science & technology, 2014, 46(6):665-670.

We isolated twelve lignans and three terpenoids were isolated from the n-hexane fraction of Schisandra chinensis extract.
METHODS AND RESULTS:
Using spectroscopic data and comparison with available literature, the following compounds were identified: (1) wuweizisu C, (2) gomisin N, (3) deoxyschisandrin, (4) gomisin A, (5) schisandrin, (6) chamigrenal, (7) schisanlactone D, (8) Methylgomisin O, (9) angeloylgomisin O, (10) (-)-gomisin L2, (11) schisandronic acid, (12) (-)-gomisin L1, (13) (+)-gomisin K3, (14) gomisin J, and (15) tigloylgomisin H. Notably, this was the first finding that compound (8) was isolated from this plant. Each compound was evaluated for its in vitro cytotoxic activities toward HL-60 (human leukemia), HeLa (human cervical carcinoma), and MCF-7 (breast cancer) cell lines.
CONCLUSIONS:
Compounds (7), (8), and (9) exhibited strong cytotoxic effects on HL-60 (IC50 7.37, 6.60, and 8.00 μM, respectively), whereas compound (6) exhibited weak cytotoxicity towards MCF-7 (IC50 30.50 μM). In addition, compound (8) showed the strongest activity towards HeLa cells (IC50 1.46 μM).

New Dibenzocyclooctadiene Lignans from Schisandra sphenanthera and Their Proinflammatory Cytokine Inhibitory Activities.[Reference: WebLink]

Zeitschrift Für Naturforschung B, 2010, 65(2):1-8.

METHODS AND RESULTS:
Investigation of the fruits of Schisandra sphenanthera led to the isolation of two new dibenzocyclooctadiene lignans, Methylgomisin O (1) and chloromethyl schisantherin B (2), together with twelve known lignans (3-14). Their structures were elucidated by using extensive spectroscopic techniques including 1D and 2D NMR spectra. Compound 2 was identified as a cyclooctadiene moiety substituted with a chloromethyl group, which is rarely found in natural products, especially in terrestrial higher plants.
CONCLUSIONS:
Among these isolates, compounds 1 and 7 exhibited considerable inhibitory activity against tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) production, and did not display any cellular toxicity against RAW264.7 cells.