Neopuerarin B

CAS# 1150314-39-8

Neopuerarin B

Catalog No. BCX0997----Order now to get a substantial discount!

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Chemical structure

Neopuerarin B

3D structure

Chemical Properties of Neopuerarin B

Cas No. 1150314-39-8 SDF Download SDF
PubChem ID 91029430.0 Appearance Powder
Formula C21H20O9 M.Wt 416.38
Type of Compound Flavonoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 8-[(2S,3R,4R,5R)-5-[(1R)-1,2-dihydroxyethyl]-3,4-dihydroxyoxolan-2-yl]-7-hydroxy-3-(4-hydroxyphenyl)chromen-4-one
SMILES C1=CC(=CC=C1C2=COC3=C(C2=O)C=CC(=C3C4C(C(C(O4)C(CO)O)O)O)O)O
Standard InChIKey ANUVQPJCPQLESC-BUQAOYBGSA-N
Standard InChI InChI=1S/C21H20O9/c22-7-14(25)20-17(27)18(28)21(30-20)15-13(24)6-5-11-16(26)12(8-29-19(11)15)9-1-3-10(23)4-2-9/h1-6,8,14,17-18,20-25,27-28H,7H2/t14-,17-,18-,20-,21+/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Neopuerarin B

Lobed Kudzuvine Root

Neopuerarin B Dilution Calculator

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Neopuerarin B Molarity Calculator

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Preparing Stock Solutions of Neopuerarin B

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.4017 mL 12.0083 mL 24.0165 mL 48.033 mL 60.0413 mL
5 mM 0.4803 mL 2.4017 mL 4.8033 mL 9.6066 mL 12.0083 mL
10 mM 0.2402 mL 1.2008 mL 2.4017 mL 4.8033 mL 6.0041 mL
50 mM 0.048 mL 0.2402 mL 0.4803 mL 0.9607 mL 1.2008 mL
100 mM 0.024 mL 0.1201 mL 0.2402 mL 0.4803 mL 0.6004 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on Neopuerarin B

New hepatoprotective isoflavone glucosides from Pueraria lobata (Willd.) Ohwi.[Pubmed:29968479]

Nat Prod Res. 2019 Dec;33(24):3485-3492.

Two new isoflavone glucosides, 3'-methoxyneopuerarin A (1) and 3'-methoxyNeopuerarin B (2), together with nine known isoflavones including puerarin (3), neopuerarin A (4), Neopuerarin B (5), daidzin (6), daidzein (7), 3'-methoxypuerarin (PG-3) (8), puerarin xyloside (9), mirificin (10), 3'-hydroxypuerarin (11) were isolated from the water extraction of the dried roots of Pueraria lobata (Willd.) Ohwi. Their structures were elucidated by the means of spectroscopic and chromatographic analysis methods. All compounds were evaluated for their hepatoprotective activity on HepG2 cells. All of them showed statistically significant hepatoprotective effect.

Isolation of two new C-glucofuranosyl isoflavones from Pueraria lobata (Wild.) Ohwi with HPLC-MS guiding analysis.[Pubmed:20419540]

J Asian Nat Prod Res. 2010 Apr;12(4):293-9.

A profiling analysis of the total isoflavone extract of the root of Pueraria lobata (Wild.) Ohwi was performed using HPLC coupled with ESI-ion trap mass spectrometry. A total of seven isoflavones were identified according to their retention times, UV, MS data, and comparing with the literature data. Among them, two proposed new compounds were isolated and their structures were determined to be 8-C-alpha-glucofuranosyl-7,4'-dihydroxyisoflavone and 8-C-beta-glucofuranosyl-7,4'-dihydroxyisoflavone, named as neopuerarin A (7) and Neopuerarin B (6), on the basis of chemical and spectral analyses.

Profiling and quantification of isoflavone-C-glycosides impurities in puerarin injection by liquid chromatography coupled to ESI-ion trap mass spectrometry.[Pubmed:19186018]

J Pharm Biomed Anal. 2009 Apr 5;49(3):843-7.

An HPLC/DAD/MS(n) method was established for the qualitative and quantitative analysis of the impurities in puerarin injection (PI), a widely used drug in China. The analytical HPLC was performed on an Agela RP-C18 column using 0.1% aqueous formic acid (v:v) and methanol as mobile phase. A total of nine impurities were detected and eight of them were identified as isoflavone-C-glycosides basing on their UV spectra and MS(n) spectra and comparing with the literature data. An HPLC method for the assay of two common impurities in the commercial PI samples, i.e., neopuerarin A and Neopuerarin B, was then established. The validation of the method, including sensitivity, linearity, precision, accuracy, was carried out. The calibration curves showed good linearity of R(2)>0.9999 and LOQ (S/N=10) were less than 3.73 ng. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 0.94%. The average recovery rates were 97.0% and 99.5%, respectively, with R.S.D. less than 1.38%. The contents of neopuerarin A and Neopuerarin B in various commercial brands of PI samples varied over the range of 0.30-1.16% and 0.42-1.66%, respectively. This is the first report on the impurities in PI.

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