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2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

CAS# 69804-59-7

2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

2D Structure

Catalog No. BCN1374----Order now to get a substantial discount!

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Quality Control of 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

Quality Control & MSDS

3D structure

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2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

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Chemical Properties of 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

Cas No. 69804-59-7 SDF Download SDF
PubChem ID 11074014 Appearance Powder
Formula C8H7NO4 M.Wt 181.1
Type of Compound Alkaloids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 2,7-dihydroxy-4H-1,4-benzoxazin-3-one
SMILES C1=CC2=C(C=C1O)OC(C(=O)N2)O
Standard InChIKey HDRDOKTYPONKPM-UHFFFAOYSA-N
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

The roots of Scoparia dulcis

Biological Activity of 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

DescriptionStandard reference

Protocol of 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

Structure Identification
Plant Physiol. 1979 Jan;63(1):9-13.

Identification of 1,4-Benzoxazin-3-ones in Maize Extracts by Gas-Liquid Chromatography and Mass Spectrometry.[Pubmed: 16660700]


METHODS AND RESULTS:
Based on spectral comparisons, three hydroxamic acids (2,4-dihydroxy-2H-1, 4-benzoxazin-3(4H)-one; 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one; and 2,4-dihydroxy-7,8-dimethoxy-2H-1,4-benzoxazin-3(4H)-one), three lactams (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one; 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one; and 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one), one benzoxazolinone (6-methoxybenzoxazolinone), and two organic acids (malic and aconitic) were identified in the extracts. In addition, one other hydroxamic acid and one other related compound were tentatively identified based on mass spectral evidence.

2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one Dilution Calculator

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2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one Molarity Calculator

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Preparing Stock Solutions of 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 5.5218 mL 27.6091 mL 55.2181 mL 110.4362 mL 138.0453 mL
5 mM 1.1044 mL 5.5218 mL 11.0436 mL 22.0872 mL 27.6091 mL
10 mM 0.5522 mL 2.7609 mL 5.5218 mL 11.0436 mL 13.8045 mL
50 mM 0.1104 mL 0.5522 mL 1.1044 mL 2.2087 mL 2.7609 mL
100 mM 0.0552 mL 0.2761 mL 0.5522 mL 1.1044 mL 1.3805 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one

Identification of 1,4-Benzoxazin-3-ones in Maize Extracts by Gas-Liquid Chromatography and Mass Spectrometry.[Pubmed:16660700]

Plant Physiol. 1979 Jan;63(1):9-13.

Gas-liquid chromatography-mass spectrometry (GLC-MS) has been used for the separation, detection, and identification of 1,4-benzoxazin-3-ones (hydroxamic acids and lactams) and benzoxazolinones found in maize (Zea mays L.) extracts. Compounds of interest were partitioned into ethyl acetate from aqueous maize seedling extracts. For analysis by GLC-MS, trimethylsilyl derivatives were prepared, chromatographed on a column of 3% OV-1, and detected in the mass spectrometer. Mass spectra were obtained for all peaks present in extracts of four maize lines. A data comparison system was developed for relating unidentified spectra to the spectra of the reference compounds. Based on spectral comparisons, three hydroxamic acids (2,4-dihydroxy-2H-1, 4-benzoxazin-3(4H)-one; 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one; and 2,4-dihydroxy-7,8-dimethoxy-2H-1,4-benzoxazin-3(4H)-one), three lactams (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one; 2,7-Dihydroxy-2H-1,4-benzoxazin-3(4H)-one; and 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one), one benzoxazolinone (6-methoxybenzoxazolinone), and two organic acids (malic and aconitic) were identified in the extracts. In addition, one other hydroxamic acid and one other related compound were tentatively identified based on mass spectral evidence.

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