CilomilastPotent PDE4 inhibitor CAS# 153259-65-5 |
- Olprinone Hydrochloride
Catalog No.:BCC1821
CAS No.:119615-63-3
- GSK256066 2,2,2-trifluoroacetic acid
Catalog No.:BCC1605
CAS No.:1415560-64-3
- CDP 840 hydrochloride
Catalog No.:BCC7814
CAS No.:162542-90-7
- Nortadalafil
Catalog No.:BCC1806
CAS No.:171596-36-4
- Rolipram
Catalog No.:BCC2282
CAS No.:61413-54-5
- Oglemilast
Catalog No.:BCC1817
CAS No.:778576-62-8
Quality Control & MSDS
Number of papers citing our products
Chemical structure
3D structure
Cas No. | 153259-65-5 | SDF | Download SDF |
PubChem ID | 151170 | Appearance | Powder |
Formula | C20H25NO4 | M.Wt | 343.42 |
Type of Compound | N/A | Storage | Desiccate at -20°C |
Synonyms | SB-207499 | ||
Solubility | DMSO : ≥ 100 mg/mL (291.19 mM) *"≥" means soluble, but saturation unknown. | ||
Chemical Name | 4-cyano-4-(3-cyclopentyloxy-4-methoxyphenyl)cyclohexane-1-carboxylic acid | ||
SMILES | COC1=C(C=C(C=C1)C2(CCC(CC2)C(=O)O)C#N)OC3CCCC3 | ||
Standard InChIKey | CFBUZOUXXHZCFB-UHFFFAOYSA-N | ||
Standard InChI | InChI=1S/C20H25NO4/c1-24-17-7-6-15(12-18(17)25-16-4-2-3-5-16)20(13-21)10-8-14(9-11-20)19(22)23/h6-7,12,14,16H,2-5,8-11H2,1H3,(H,22,23) | ||
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. |
Description | Cilomilast is a potent inhibitor of phosphodiesterase 4 (PDE4) with IC50 value of 110 nM. | |||||
Targets | PDE4 | |||||
IC50 | 110 nM |
Cell experiment [1]: | |
Cell lines | MCS cell lines |
Preparation method | The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months. |
Reacting condition | 11 d; 40 μM |
Applications | The results demonstrate that inhibition of PDE (Cilomilast) enhances ALP expression in MSCs via the cAMP pathway. The increase in the level of ALP activity is dependent on the dose of cilomilast. To study the effect of the inducers on MSC differentiation at similar proliferation rates, we treated MCSs, except those cultured in osteogenic medium, with 1% DMSO. We compared MSCs cultured for 11 days in the presence of different inducers with MSCs cultured in osteogenic medium in order to quantify the osteogenetic effects of the inducers. We found that the ALP activity levels of MCSs treated with a combination of PDE4 inhibitor (40 μM) and BMP-2 (300 ng/mL) were almost double the ALP activity level of MSCs treated with osteogenic medium, suggesting that the mineralisation process is more rapid. |
Animal experiment [2]: | |
Animal models | Female C57BL/6 mice |
Dosage form | Cilomilast 0.05%; ocular surface instillation three times per day over a period of 7 days. |
Application | Real-time PCR was used to quantify the expression of transcripts encoding IL-1α, IL-1β, and TNF-α in the corneas and conjunctivae of DED-induced mice. Treatment with topical cilomilast significantly decreased the corneal expression of TNF-α as compared with the vehicle-treated group. Compared with the DED-untreated corneas, treatment with cilomilast significantly reduced IL-1α and TNF-α expression. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1] Munisso M C, Kang J H, Tsurufuji M, et al. Cilomilast enhances osteoblast differentiation of mesenchymal stem cells and bone formation induced by bone morphogenetic protein 2[J]. Biochimie, 2012, 94(11): 2360-2365. [2] Sadrai Z, Stevenson W, Okanobo A, et al. PDE4 inhibition suppresses IL-17–associated immunity in dry eye disease[J]. Investigative ophthalmology & visual science, 2012, 53(7): 3584-3591. |
Cilomilast Dilution Calculator
Cilomilast Molarity Calculator
1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
1 mM | 2.9119 mL | 14.5594 mL | 29.1189 mL | 58.2377 mL | 72.7972 mL |
5 mM | 0.5824 mL | 2.9119 mL | 5.8238 mL | 11.6475 mL | 14.5594 mL |
10 mM | 0.2912 mL | 1.4559 mL | 2.9119 mL | 5.8238 mL | 7.2797 mL |
50 mM | 0.0582 mL | 0.2912 mL | 0.5824 mL | 1.1648 mL | 1.4559 mL |
100 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.5824 mL | 0.728 mL |
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. |
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
Cilomilast, also known as SB-207499 or Ariflo, is a potent second generation inhibitor of type 4 phosphodiesterase (PDE4), an enzyme metabolizing cellular cyclic adenosine monophosphate (cAMP) which acts as a second messenger to disrupt the function of inflammatory cell and induce airway smooth muscle relaxation. Cilomilast is currently used for the treatment of chronic obstructive pulmonary disease (COPD) due to its strong anti-inflammatory activity as well as inhibitory effects against the release of neutrophil chemoattractants (such as tumor necrosis factor TNF- α, interleukin IL-8 and granulocyte-macrophage colony stimulating factor GM-CSF) and suppression of the recruitment of neutrophils into tissues and the LTB4 production.
Reference
M Profita, G Chiappara, F Mirabella, RCDi Giorgi, L Chimenti, G Costanzo, L Riccobono, V Bellia, J Bousquet, and A M Vignola. Effect of cilomilast (Ariflo) on TNF-α, IL-8, and GM-CSF release by airway cells of patients with COPD. Thorax 2003; 58: 573-579
Barry D. Zussman, Lisa J. Benincosa, Dawn M Webber, David j. Clark, Hugh Cowley, John Kelly, Robert D. Murdoch, James Upward, Peter Wyld, Andreas Port and Hermann Fuder. An overview of the pharmacokinetics of cilomilast (Ariflo), a new, orally active phosphodiesterase 4 inhibitor, in healthy young and elderly volunteers. J Clin Pharmacol 2001; 41: 950-958
- Taxayunnansin A
Catalog No.:BCN1685
CAS No.:153229-31-3
- Thiamphenicol
Catalog No.:BCC4736
CAS No.:15318-45-3
- N-Methyltaxol C
Catalog No.:BCN7343
CAS No.:153083-53-5
- Diclofenac
Catalog No.:BCC5249
CAS No.:15307-86-5
- Diclofenac Sodium
Catalog No.:BCC4439
CAS No.:15307-79-6
- SR 140333
Catalog No.:BCC6098
CAS No.:153050-21-6
- Precursor of cefcapene diisopropylanmine salt
Catalog No.:BCC9127
CAS No.:153012-37-4
- Serotonin HCl
Catalog No.:BCC4715
CAS No.:153-98-0
- H-D-Trp-OH
Catalog No.:BCC3117
CAS No.:153-94-6
- 2-Aminofluorene
Catalog No.:BCC8549
CAS No.:153-78-6
- Rutin
Catalog No.:BCN1684
CAS No.:153-18-4
- Guajadial F
Catalog No.:BCN6437
CAS No.:1529775-08-3
- ML355
Catalog No.:BCC8060
CAS No.:1532593-30-8
- 4,4'-Bis(2-benzoxazolyl)stilbene
Catalog No.:BCC8656
CAS No.:1533-45-5
- DFB
Catalog No.:BCC7130
CAS No.:15332-10-2
- SCR7
Catalog No.:BCC3978
CAS No.:1533426-72-0
- Ginkgolide K
Catalog No.:BCN8209
CAS No.:153355-70-5
- 7-Epi-docetaxel
Catalog No.:BCC5411
CAS No.:153381-68-1
- Taxol C
Catalog No.:BCN6941
CAS No.:153415-45-3
- Taxcultine
Catalog No.:BCN6948
CAS No.:153415-46-4
- Gavestinel
Catalog No.:BCC7340
CAS No.:153436-38-5
- AG-1478
Catalog No.:BCC3717
CAS No.:153436-53-4
- PD153035 hydrochloride
Catalog No.:BCC3617
CAS No.:153436-54-5
- WHI-P180 hydrochloride
Catalog No.:BCC4243
CAS No.:153437-55-9
Cilomilast enhances osteoblast differentiation of mesenchymal stem cells and bone formation induced by bone morphogenetic protein 2.[Pubmed:22706281]
Biochimie. 2012 Nov;94(11):2360-5.
A rapid and efficient method to stimulate bone regeneration would be useful in orthopaedic stem cell therapies. Rolipram is an inhibitor of phosphodiesterase 4 (PDE4), which mediates cyclic adenosine monophosphate (cAMP) degradation. Systemic injection of rolipram enhances osteogenesis induced by bone morphogenetic protein 2 (BMP-2) in mice. However, there is little data on the precise mechanism, by which the PDE4 inhibitor regulates osteoblast gene expression. In this study, we investigated the combined ability of BMP-2 and Cilomilast, a second-generation PDE4 inhibitor, to enhance the osteoblastic differentiation of mesenchymal stem cells (MSCs). The alkaline phosphatase (ALP) activity of MSCs treated with PDE4 inhibitor (Cilomilast or rolipram), BMP-2, and/or H89 was compared with the ALP activity of MSCs differentiated only by osteogenic medium (OM). Moreover, expression of Runx2, osterix, and osteocalcin was quantified using real-time polymerase chain reaction (RT-PCR). It was found that Cilomilast enhances the osteoblastic differentiation of MSCs equally well as rolipram in primary cultured MSCs. Moreover, according to the H89 inhibition experiments, Smad pathway was found to be an important signal transduction pathway in mediating the osteogenic effect of BMP-2, and this effect is intensified by an increase in cAMP levels induced by PDE4 inhibitor.
Repurposing human PDE4 inhibitors for neglected tropical diseases: design, synthesis and evaluation of cilomilast analogues as Trypanosoma brucei PDEB1 inhibitors.[Pubmed:25127163]
Bioorg Med Chem Lett. 2014 Sep 1;24(17):4084-9.
A medicinal chemistry exploration of the human phosphodiesterase 4 (hPDE4) inhibitor Cilomilast (1) was undertaken in order to identify inhibitors of phosphodiesterase B1 of Trypanosoma brucei (TbrPDEB1). T. brucei is the parasite which causes African sleeping sickness, a neglected tropical disease that affects thousands each year, and TbrPDEB1 has been shown to be an essential target of therapeutic relevance. Noting that 1 is a weak inhibitor of TbrPDEB1, we report the design and synthesis of analogs of this compound, culminating in 12b, a sub-micromolar inhibitor of TbrPDEB1 that shows modest inhibition of T. brucei proliferation.
Comparison of the anti-inflammatory effects of Cilomilast, Budesonide and a p38 Mitogen activated protein kinase inhibitor in COPD lung tissue macrophages.[Pubmed:23148608]
BMC Pharmacol Toxicol. 2012 Nov 13;13:15.
Chronic Obstructive Pulmonary Disease (COPD) is a disease characterized by a largely irreversible airflow obstruction and a persistent, excessive inflammatory response. Alveolar macrophages (AMs) are increased in the lungs of COPD patients, and act as orchestrators of the inflammatory response, releasing a range of mediators to coordinate recruitment and activation of leukocytes. Attempts to treat the inflammatory component of COPD with anti-inflammatory drugs such as steroids has met with limited success. In this study, we compared the ability of the phosphodiesterase IV (PDEIV) inhibitor Cilomilast, the steroid Budesonide, and the p38 mitogen activated protein kinase inhibitor BIRB-796 to inhibit tumour necrosis factor alpha (TNFalpha) and interleukin 6 (IL-6) releases from AMs isolated from COPD lung transplant tissue. All studies were carried out with appropriate ethical approval and written, informed consent was obtained from each subject. Cilomilast had little effect on cytokine release from AMs. There was considerable variability in the responsiveness of AMs to Budesonide, with a subset of AMs responding poorly to Budesonide. BIRB-796 inhibited TNFalpha release from all AM donors, including those that responded poorly to steroids. Treatment with BIRB-796 and Budesonide together gave an additive decrease in TNFa release. These results suggest that a p38 inhibitor may provide advantages over existing anti-inflammatory treatments for COPD, either as an add-on to existing therapy, or to treat patients who respond poorly to steroids.
Cilomilast counteracts the effects of cigarette smoke in airway epithelial cells.[Pubmed:21382614]
Cell Immunol. 2011;268(1):47-53.
Cigarette smoke extracts (CSE) alter TLR4 expression and activation in bronchial epithelial cells. Cilomilast, a phosphodiesterase-4 inhibitor, inhibits cigarette smoke-induced neutrophilia. This study was aimed to explore whether Cilomilast, in a human bronchial epithelial cell line (16-HBE), counteracted CSE effects. In particular, TLR4 expression, IP-10 and IL-8 release, lymphocyte and neutrophil chemotactic activity and ERK and IkBa phosphorylation in CSE and LPS-stimulated 16-HBE were assessed. CSE increased TLR4 expression, reduced IP-10 release and lymphocyte chemotactic activity and increased IL-8 release and neutrophil chemotactic activity. Cilomilast reduced TLR4 expression, IL-8 release and neutrophil chemotactic activity as well as it increased IP-10 release and lymphocyte chemotactic activity. All these Cilomilast mediated effects were associated with a reduced ERK1/2 and with an increased IkBa phosphorylation. In conclusion, the present study provides compelling evidences that Cilomilast may be considered a possible valid therapeutic option in controlling inflammatory processes present in smokers.