DaucosterolCAS# 474-58-8 |
2D Structure
Quality Control & MSDS
3D structure
Package In Stock
Number of papers citing our products
Cas No. | 474-58-8 | SDF | Download SDF |
PubChem ID | 5742590 | Appearance | White powder |
Formula | C35H60O6 | M.Wt | 576.9 |
Type of Compound | Steroids | Storage | Desiccate at -20°C |
Synonyms | Eleutheroside A; β-Sitosterol β-D-glucoside;Sitogluside | ||
Solubility | DMSO : 7.9 mg/mL (13.70 mM; Need ultrasonic and warming) | ||
Chemical Name | (2R,3R,4S,5S,6R)-2-[[(3S,8S,9S,10R,13R,14S,17R)-17-[(2R,5R)-5-ethyl-6-methylheptan-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl]oxy]-6-(hydroxymethyl)oxane-3,4,5-triol | ||
SMILES | CCC(CCC(C)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)OC5C(C(C(C(O5)CO)O)O)O)C)C)C(C)C | ||
Standard InChIKey | NPJICTMALKLTFW-OFUAXYCQSA-N | ||
Standard InChI | InChI=1S/C35H60O6/c1-7-22(20(2)3)9-8-21(4)26-12-13-27-25-11-10-23-18-24(14-16-34(23,5)28(25)15-17-35(26,27)6)40-33-32(39)31(38)30(37)29(19-36)41-33/h10,20-22,24-33,36-39H,7-9,11-19H2,1-6H3/t21-,22-,24+,25+,26-,27+,28+,29-,30-,31+,32-,33-,34+,35-/m1/s1 | ||
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. |
Description | Daucosterol has neuroprotective activity, it has proliferation-enhancing activity for neural stem cells (NSCs), may be involved in IGF1-AKT pathway, and it as an efficient and inexpensive growth factor alternative that could be potentially developed as a medicine for ischemic stroke treatment, can significantly reduce neuronal loss.Daucosterol exhibits moderate antibacterial activity against Bacillus subtilis and Staphylococcus aureus; it has anti-cancer and apoptotic effects in human colon cancer cell line HCT-116, at different doses induces cell cycle arrest at sub-G1 phase of the cell cycle. |
Targets | IGF-1R | Akt | Bcl-2/Bax | Caspase | GSK-3 | Antifection | IFN-γ | IL Receptor | ROS | gp120/CD4 |
In vitro | Daucosterol promotes the proliferation of neural stem cells.[Pubmed: 24333794]J Steroid Biochem Mol Biol. 2014 Mar;140:90-9.Neural stem cells (NSCs) are self-regenerating cells, but their regenerative capacity is limited.
Daucosterol protects neurons against oxygen-glucose deprivation/reperfusion-mediated injury by activating IGF1 signaling pathway.[Pubmed: 25864625]J Steroid Biochem Mol Biol. 2015 Apr 9;152:45-52.We previously reported that Daucosterol (a sterolin) up-regulates the expression of insulin-like growth factor I (IGF1)(1) protein in neural stem cells.
A novel daucosterol derivative and antibacterial activity of compounds from Arctotis arctotoides.[Pubmed: 17680499]Nat Prod Res. 2007 Aug;21(10):889-96.Arctotis arctotoides is a perennial herb used medicinally for the treatment of various ailments in the Eastern Cape, South Africa. Different extracts of the plant were investigated for their antimicrobial constituents.
|
In vivo | Immunoregulatory activity by daucosterol, a beta-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice.[Pubmed: 17335944 ]Vaccine. 2007 May 10;25(19):3834-40.
|
Cell Research | Daucosterol inhibits colon cancer growth by inducing apoptosis, inhibiting cell migration and invasion and targeting caspase signalling pathway.[Reference: WebLink]Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner.[Pubmed: 26209138]Life Sci. 2015 Sep 15;137:37-43.This study aims to evaluate the anti-cancer effect of Daucosterol and explore its possible mechanism.
Bangladesh Journal of Pharmacology, 2016, 11(2):395.The aim of the present investigation was to examine and demonstrate in detail the anti-cancer and apoptotic effects of Daucosterol in human colon cancer cell line HCT-116.
|
Daucosterol Dilution Calculator
Daucosterol Molarity Calculator
1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
1 mM | 1.7334 mL | 8.667 mL | 17.334 mL | 34.6681 mL | 43.3351 mL |
5 mM | 0.3467 mL | 1.7334 mL | 3.4668 mL | 6.9336 mL | 8.667 mL |
10 mM | 0.1733 mL | 0.8667 mL | 1.7334 mL | 3.4668 mL | 4.3335 mL |
50 mM | 0.0347 mL | 0.1733 mL | 0.3467 mL | 0.6934 mL | 0.8667 mL |
100 mM | 0.0173 mL | 0.0867 mL | 0.1733 mL | 0.3467 mL | 0.4334 mL |
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. |
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
Daucosterol is a natural sterolin. IC50 value: Target: In vitro: In the study of the effects of daucosterol on the survival of cultured cortical neurons after neurons were subjected to oxygen and glucose deprivation and simulated reperfusion (OGD/R)(2), the results showed that post-treatment of daucosterol significantly reduced neuronal loss, as well as apoptotic rate and caspase-3 activity, displaying the neuroprotective activity. We also found that daucosterol increased the expression level of IGF1 protein, diminished the down-regulation of p-AKT(3) and p-GSK-3β(4), thus activating the AKT(5) signal pathway [1]. Cell counting kit-8 (CCK-8) assay showed that daucosterol significantly increased the quantity of viable cells and the effectiveness of daucosterol was similar to that of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) [2]. Daucosterol inhibits the proliferation of human breast cancer cell line MCF-7 and gastric cancer cell lines MGC803, BGC823 and AGS in a dose-dependent manner. Furthermore, daucosterol inhibits murine hepatoma H22 cell growth in ICR mice. Daucosterol treatment induces intracellular ROS generation and autophagy, but not apoptotic cell death. Treatment with ROS scavenger GSH (reduced glutathione), NAC (N-acetyl-l-cysteine) or autophagy inhibitor 3-Methyladenine (3-MA) counteracted daucosterol-induced autophagy and growth inhibition in BGC823 and MCF-7 cancer cells [3]. In vivo:
References:
[1]. Jiang LH, et al. Daucosterol protects neurons against oxygen-glucose deprivation/reperfusion-mediated injury by activating IGF1 signaling pathway. J Steroid Biochem Mol Biol. 2015 Aug;152:45-52.
[2]. Jiang LH, et al. Daucosterol promotes the proliferation of neural stem cells. J Steroid Biochem Mol Biol. 2014 Mar;140:90-9.
[3]. Zhao C, et al. Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner. Life Sci. 2015 Sep 15;137:37-43.
- Reserpin N-oxide
Catalog No.:BCN3493
CAS No.:474-48-6
- Citrostadienol
Catalog No.:BCN7357
CAS No.:474-40-8
- Chenodeoxycholic acid
Catalog No.:BCN2620
CAS No.:474-25-9
- Brazilin
Catalog No.:BCN5529
CAS No.:474-07-7
- Forskolin G
Catalog No.:BCN5527
CAS No.:473981-11-2
- Lersivirine
Catalog No.:BCC1698
CAS No.:473921-12-9
- Boc-Tyr(tBu)-OH
Catalog No.:BCC3462
CAS No.:47375-34-8
- SCH 563705
Catalog No.:BCC1933
CAS No.:473728-58-4
- SCH 527123
Catalog No.:BCC1932
CAS No.:473727-83-2
- AMG 487
Catalog No.:BCC5140
CAS No.:473719-41-4
- Boc-Trp(For)-OH
Catalog No.:BCC3456
CAS No.:47355-10-2
- Carpinontriol B
Catalog No.:BCN8113
CAS No.:473451-73-9
- Campestanol
Catalog No.:BCN3890
CAS No.:474-60-2
- Campesterol
Catalog No.:BCN3181
CAS No.:474-62-4
- Brassicasterol
Catalog No.:BCN2613
CAS No.:474-67-9
- CAL-130 Racemate
Catalog No.:BCC1442
CAS No.:474012-90-3
- N-Methylsarpagine methosalt
Catalog No.:BCN5530
CAS No.:47418-70-2
- Monomethyl auristatin E
Catalog No.:BCC1775
CAS No.:474645-27-7
- 2,16-Kauranediol 2-O-beta-D-allopyranoside
Catalog No.:BCN1436
CAS No.:474893-07-7
- ST 91
Catalog No.:BCC7436
CAS No.:4749-61-5
- SB 657510
Catalog No.:BCC7713
CAS No.:474960-44-6
- H-N-Me-Pro-OH
Catalog No.:BCC3351
CAS No.:475-11-6
- (+)-Isocorynoline
Catalog No.:BCN2361
CAS No.:475-67-2
- Liriodenine
Catalog No.:BCN5532
CAS No.:475-75-2
Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner.[Pubmed:26209138]
Life Sci. 2015 Sep 15;137:37-43.
AIMS: This study aims to evaluate the anti-cancer effect of Daucosterol and explore its possible mechanism. MAIN METHODS: MTT and colony formation assay were performed to determine the effect of Daucosterol on cancer cell proliferation in vitro. H22 allograft model was used for the assessment of its anti-cancer activity in vivo. Intracellular generation of reactive oxygen species (ROS) was measured using DCFH-DA probe with flow cytometry system and a laser scanning confocal microscope. LC3 (microtubule-associated protein 1 light chain 3)-II conversion was monitored with immunofluorescence and immunoblotting to demonstrate Daucosterol-induced autophagy. KEY FINDINGS: We found that Daucosterol inhibits the proliferation of human breast cancer cell line MCF-7 and gastric cancer cell lines MGC803, BGC823 and AGS in a dose-dependent manner. Furthermore, Daucosterol inhibits murine hepatoma H22 cell growth in ICR mice. Daucosterol treatment induces intracellular ROS generation and autophagy, but not apoptotic cell death. Treatment with ROS scavenger GSH (reduced glutathione), NAC (N-acetyl-l-cysteine) or autophagy inhibitor 3-Methyladenine (3-MA) counteracted Daucosterol-induced autophagy and growth inhibition in BGC823 and MCF-7 cancer cells. SIGNIFICANCE: Daucosterol inhibits cancer cell proliferation by inducing autophagy through ROS-dependent manner and could be potentially developed as an anti-cancer agent.
A novel daucosterol derivative and antibacterial activity of compounds from Arctotis arctotoides.[Pubmed:17680499]
Nat Prod Res. 2007 Aug;21(10):889-96.
Arctotis arctotoides is a perennial herb used medicinally for the treatment of various ailments in the Eastern Cape, South Africa. Different extracts of the plant were investigated for their antimicrobial constituents. This led to the isolation and identification of a new Daucosterol derivative 3-O-[beta-D-(6'-nonadeanoate)glucopyranosyl]-beta-sitosterol and seven known compounds namely: serratagenic acid, stigmasterol, Daucosterol, zaluzanin D, dehydrocostuslactone, nepetin, and pedalitin. The structures of the compounds were elucidated on the basis of spectral analysis, including homo and hetero nuclear correlation NMR experiments (COSY, NOESY, HMQC, HMBC) and mass spectra as well as by comparison with available data in the literature. The compounds exhibited antibacterial activity except stigmasterol, Daucosterol and dehydrocostuslactone. Nepetin was the most active against Bacillus subtilis and Staphylococcus aureus with the minimum inhibitory concentrations of 4 microg mL( - 1) and 31 microg mL( - 1), respectively, while others exhibited moderate activity.
Immunoregulatory activity by daucosterol, a beta-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice.[Pubmed:17335944]
Vaccine. 2007 May 10;25(19):3834-40.
In the present study, we investigated immunomodulatory effect of Daucosterol, a beta-sitosterol glycoside, against disseminated candidiasis caused by Candida albicans. Results showed that direct interaction of Daucosterol with C. albicans yeast cells resulted in no growth-inhibition by in vitro susceptibility analysis. In contrast, mice given Daucosterol (DS) intraperitoneally before intravenous challenge with live C. albicans yeast cells survived longer than DS-untreated control mice against disseminated candidiasis (P<0.05). By assessment of the fungal CFU in kidneys, DS-treated mice before the challenge developed about 81% fewer kidney CFU than untreated controls. This protection was removable by pretreatment of mice with anti-CD4+ antibody before the DS-treatment and challenge with the yeast. However, the protection was transferable by the CD4+ T cells from DS-treated mice not infected with the yeast. ELISA analysis revealed there were predominant production of IFNgamma and IL-2 cytokines as compared to IL-4, and IL-10 productions in DS-treated mice. By treatment of DS-given mice with anti-mouse IFNgamma, the protection was also abolished. Our studies show that DS protects mice against disseminated candidiasis by the CD4+ Th1 immune response.
Daucosterol protects neurons against oxygen-glucose deprivation/reperfusion-mediated injury by activating IGF1 signaling pathway.[Pubmed:25864625]
J Steroid Biochem Mol Biol. 2015 Aug;152:45-52.
We previously reported that Daucosterol (a sterolin) up-regulates the expression of insulin-like growth factor I (IGF1)(1) protein in neural stem cells. In this study, we investigated the effects of Daucosterol on the survival of cultured cortical neurons after neurons were subjected to oxygen and glucose deprivation and simulated reperfusion (OGD/R)(2), and determined the corresponding molecular mechanism. The results showed that post-treatment of Daucosterol significantly reduced neuronal loss, as well as apoptotic rate and caspase-3 activity, displaying the neuroprotective activity. We also found that Daucosterol increased the expression level of IGF1 protein, diminished the down-regulation of p-AKT(3) and p-GSK-3beta(4), thus activating the AKT(5) signal pathway. Additionally, it diminished the down-regulation of the anti-apoptotic proteins Mcl-1(6) and Bcl-2(7), and decreased the expression level of the pro-apoptotic protein Bax(8), thus raising the ratio of Bcl-2/Bax. The neuroprotective effect of Daucosterol was inhibited in the presence of picropodophyllin (PPP)(9), the inhibitor of insulin-like growth factor I receptors (IGF1R)(10). Our study provided information about Daucosterol as an efficient and inexpensive neuroprotectants, to which the IGF1-like activity of Daucosterol contributes. Daucosterol could be potentially developed as a medicine for ischemic stroke treatment.
Daucosterol promotes the proliferation of neural stem cells.[Pubmed:24333794]
J Steroid Biochem Mol Biol. 2014 Mar;140:90-9.
Neural stem cells (NSCs) are self-regenerating cells, but their regenerative capacity is limited. The present study was conducted to investigate the effect of Daucosterol (a sterolin) on the promotion of NSC proliferation and determine the corresponding molecular mechanism. Results of cell counting kit-8 (CCK-8) assay showed that Daucosterol significantly increased the quantity of viable cells and the effectiveness of Daucosterol was similar to that of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Flow cytometry detection of CFSE-labeled (CFSE, carboxyfluorescein diacetate succinimidyl ester) NSCs showed that Div Index (or the average number of cell divisions) and % Divided (or the percentage of cells that divided at least once) of the cells were increased, indicating that Daucosterol increased the percentage of NSCs re-entering the cell cycle. mRNA microarray analysis showed that 333 genes that are mostly involved in the mitotic cell cycle were up-regulated. By contrast, 627 genes that are mostly involved in differentiation were down-regulated. In particular, insulin-like growth factor I (IGF1) was considered as an important regulatory gene that functionally promoted NSC proliferation, and the increased expression of IGF1 protein was validated by ELISA. In addition, the phosphorylation of AKT was increased, indicating that the proliferation-enhancing activity of Daucosterol may be involved in IGF1-AKT pathway. Our study provided information about Daucosterol as an efficient and inexpensive growth factor alternative that could be used in clinical medicine and research applications.