DoronenineCAS# 74217-57-5 |
Quality Control & MSDS
3D structure
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Number of papers citing our products
| Cas No. | 74217-57-5 | SDF | Download SDF |
| PubChem ID | 6440559 | Appearance | Powder |
| Formula | C18H25NO5 | M.Wt | 335.40 |
| Type of Compound | Alkaloids | Storage | Desiccate at -20°C |
| Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
| SMILES | CC1CC(=CC(=O)OC2CCN3C2C(=CC3)COC(=O)C1(C)O)C | ||
| Standard InChIKey | VDHBZYVHRJQOLV-JHKFPADBSA-N | ||
| Standard InChI | InChI=1S/C18H25NO5/c1-11-8-12(2)18(3,22)17(21)23-10-13-4-6-19-7-5-14(16(13)19)24-15(20)9-11/h4,9,12,14,16,22H,5-8,10H2,1-3H3/b11-9+/t12-,14-,16-,18-/m1/s1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
| Description | 1. Doronenine shows moderate cytotoxicity on cancerous U-937 cells. |
Doronenine Dilution Calculator
Doronenine Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.9815 mL | 14.9076 mL | 29.8151 mL | 59.6303 mL | 74.5379 mL |
| 5 mM | 0.5963 mL | 2.9815 mL | 5.963 mL | 11.9261 mL | 14.9076 mL |
| 10 mM | 0.2982 mL | 1.4908 mL | 2.9815 mL | 5.963 mL | 7.4538 mL |
| 50 mM | 0.0596 mL | 0.2982 mL | 0.5963 mL | 1.1926 mL | 1.4908 mL |
| 100 mM | 0.0298 mL | 0.1491 mL | 0.2982 mL | 0.5963 mL | 0.7454 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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In vitro chemo-preventative activity of Crotalaria agatiflora subspecies agatiflora Schweinf.[Pubmed:22041105]
J Ethnopharmacol. 2011 Dec 8;138(3):748-55.
ETHNOPHARMACOLOGICAL RELEVANCE: Crotalaria species have been widely used in Chinese traditional medicine to treat several types of internal cancers. Crotalaria agatiflora is used as a medicinal plant in several African countries for the treatment of bacterial and viral infections as well as for cancer. MATERIALS AND METHODS: Water and ethanol extracts of the leaves of Crotalaria agatiflora were evaluated for cytotoxicity on four cancerous and one noncancerous cell lines, using XTT (Sodium 3'-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate) colorimetric assay. Antioxidant activity was determined using DPPH (1,1-diphenyl-2-picryl hydrazyl). Light microscopy (eosin and haematoxylin staining) and flow cytometry (Annexin-V and propidium iodide) were used to evaluate the mechanism of action of the ethanol extract and one of the isolated compounds. RESULTS: The 50% inhibitory concentration (IC(50)) of the ethanol extract was found to be 73.9 mug/mL against leukemic U-937 cells. Good antioxidant activity (IC(50)=18.89 mug/mL) of the ethanol extract indicated the potential of Crotalaria agatiflora as chemo-preventative supplement. A bioassay guided fractionation of the ethanol extract led to the isolation of two pure compounds, namely madurensine and Doronenine. Madurensine and Doronenine showed moderate cytotoxicity on cancerous U-937 cells (IC(50) values: 47.97 and 29.57 M respectively). The crude extract treated U-937 cells showed definite signs of cell death during light microscopic investigation, while little apoptosis (10-20%) and necrosis (<2%) were detected in cells treated with the extract or madurensine. CONCLUSIONS: The results indicated that Crotalaria agatiflora possesses potential chemopreventative and therapeutic properties. The exact mechanism of action should still be determined in future studies. It is hypothesized that the ethanolic extract as well as madurensine induces autophagy, which in prolonged circumstances may lead to autophagic cell death.
