Lincomycin

Implementation and Validation of an Analytical Method for Lincomycin Determination in Feathers and Edible Tissues of Broiler Chickens by Liquid Chromatography Tandem Mass Spectrometry.[Pubmed:30931158]

J Anal Methods Chem. 2019 Feb 25;2019:4569707.

Recent studies have detected different antimicrobial residues in broiler chicken feathers, where they persisted for longer periods of time and at greater concentrations than in edible tissues. However, until today, Lincomycin behaviour in this nonedible tissue has not been assessed yet. Considering this, an analytical methodology to detect and quantify this antibiotic concentration in feathers, muscle, and liver tissues from broiler chickens was implemented and in-house validated. The methodology will allow the determination of the bioaccumulation of this highly persistent antibiotic in feathers of treated birds. For this purpose, 98% Lincomycin and 95% Lincomycin D3 standards were used. Methanol was selected as the extraction solvent, and Chromabond(R) Florisil(R) cartridges were used for the clean-up stage. The separation of analytes was performed through the analytical column SunFire C18 with a running time of 4 minutes, and the instrumental analysis was performed through an LC-MS/MS, with a liquid chromatograph Agilent(R) 1290 Infinity, coupled to an AB SCIEX(R) API 5500 mass spectrometer. An internal protocol for an in-house validation was designed based on recommendations from Commission Decision 2002/657/EC and the Guidance document on the estimation of limit of detection and limit of quantification for measurements in the field of contaminants in feed and food. The average retention time for Lincomycin was 2.255 min (for quantifier ion, 126.0). The calibration curves showed a coefficient of determination (r (2)) greater than 0.99 for all matrices, while recovery levels ranged between 98% and 101%. The limit of detection (LOD) calculated was of 19, 22, and 10 mug.kg(-1), and the limit of quantification (LOQ) was of 62, 73, and 34 mug.kg(-1) in feathers, muscle, and liver, respectively. This method detects Lincomycin in the studied matrices, confidently and accurately, as it is required for designing analytical studies of drug residues in edible and nonedible tissues, such as feathers.

Curcumin solid dispersion-loaded in situ hydrogels for local treatment of injured vaginal bacterial infection and improvement of vaginal wound healing.[Pubmed:30887519]

J Pharm Pharmacol. 2019 Mar 18.

OBJECTIVES: Injured vaginal infection is detrimental to women. A curcumin hydrogel was studied for local treatment of injured vaginal infection. METHODS: Curcumin solid dispersions (CSDs) were prepared from polyvinyl pyrrolidone and characterized by differential scanning calorimetry and an X-ray diffraction method. An in situ hydrogel CSD hydrogel (CSDG) was prepared with CSD/poloxamers and characterized. In vitro curcumin release and antibacterial effects of CSDs, CSDGs and curcumin were compared. The therapeutic effect of the CSDGs and Lincomycin/Lidocaine Gel was explored after intravaginal administration on the injured rat vaginal infection models. KEY FINDINGS: Curcumin was amorphous in CSDs where curcumin rapidly released in simulated vaginal fluids. However, CSDGs showed sustained release. CSDGs quickly formed gels in the vagina. CSDGs showed high in vivo anti-Escherichia coli or Staphylococcus aureus effect though weak in vitro effect. The recovery of vaginal microenvironment and improvement of intravaginal Lactobacillus growth may be the major reason. Furthermore, CSDGs remarkably improved vaginal wound healing by alleviating inflammation and restoring vaginal epidermal tissues compared with the Lincomycin/Lidocaine Gel. CONCLUSION: CSDGs are a promising topical formulation for local treatment of vaginal bacterial infection and improvement of vaginal wound healing.

[The sensitivity to antibiotics of Corynebacterium non diphtheriae isolated in hospitals of Rostov-on-Don and the Rostovskaia oblast.][Pubmed:30802399]

Klin Lab Diagn. 2017;62(8):502-506.

The purpose of study is to analyze sensitivity of various species Corynebacterium non diphtheriasmall ie, Cyrillic to antibacterial pharmaceuticals. The strains of C. non diphtheriae are separated from patients with pathology of respiratory and urogenital tract and also from individuals subjected to preventive examination. The sensitivity to antibacterial pharmaceuticals was determined using technique of serial dilution in fluid growth medium on the basis values of minimal inhibitory concentration (mg per l). It is established that the most efficient antibacterial pharmaceuticals in the case of strains C. non diphtheriae proved to be Vancomycin, Cefazolin and Cefotaxime in general. In case of such particular species as capital ES, Cyrillic. pseudodiphtheriticum -- Cefazolin, Cefotaxime and Gentamycin; capital ES, Cyrillic.pseudotuberculosis -- Vancomycin, Cefazolin, Cefotaxime and Gentamycin; C. xerosis -- Cefotaxime; capital ES, Cyrillic.striatum -- Cefazolin and small i, Cyrillic Rifampicin. The least efficiency was manifested for strains C. non diphtheriae by Benzylpenicillin and Lincomycin in general. In case of such particular species as capital ES, Cyrillic. pseudodiphtheriticum and capital ES, Cyrillic. pseudotuberculosis -- Lincomycin and Erythromycin; C. xerosis and capital ES, Cyrillic. striatum -- Benzylpenicillin, Lincomycin and Erythromycin. In case of various species of C. non diphtheriae capital ES, Cyrillicephalosporins (Cefotaxime and Cefazolin) can be recommended as pharmaceuticals of choice and Gentamycin and Vancomycin as reserve pharmaceuticals.