MNI-caged-D-aspartateCaged D-aspartate; EAAT substrate CAS# 845555-94-4 |
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Quality Control & MSDS
Number of papers citing our products
Chemical structure
3D structure
| Cas No. | 845555-94-4 | SDF | Download SDF |
| PubChem ID | 56972233 | Appearance | Powder |
| Formula | C13H15N3O6 | M.Wt | 309.27 |
| Type of Compound | N/A | Storage | Desiccate at -20°C |
| Synonyms | 4-Methoxy-7-nitroindolinyl-caged-<span class="DL">D</span>-aspartate | ||
| Solubility | Soluble to 5 mM in water and to 10 mM in DMSO | ||
| Chemical Name | (2R)-2-amino-4-(4-methoxy-7-nitro-2,3-dihydroindol-1-yl)-4-oxobutanoic acid | ||
| SMILES | COC1=C2CCN(C2=C(C=C1)[N+](=O)[O-])C(=O)CC(C(=O)O)N | ||
| Standard InChIKey | ATNVWKDWOLQDKH-MRVPVSSYSA-N | ||
| Standard InChI | InChI=1S/C13H15N3O6/c1-22-10-3-2-9(16(20)21)12-7(10)4-5-15(12)11(17)6-8(14)13(18)19/h2-3,8H,4-6,14H2,1H3,(H,18,19)/t8-/m1/s1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
| Description | D-aspartate caged with the photosensitive 4-methoxy-7-nitroindolinyl group. Photolyzed by UV light with a quantum efficiency of 0.09 at pH 7.4. Agonist at NMDA receptors and EAAT substrate. |
MNI-caged-D-aspartate Dilution Calculator
MNI-caged-D-aspartate Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 3.2334 mL | 16.1671 mL | 32.3342 mL | 64.6684 mL | 80.8355 mL |
| 5 mM | 0.6467 mL | 3.2334 mL | 6.4668 mL | 12.9337 mL | 16.1671 mL |
| 10 mM | 0.3233 mL | 1.6167 mL | 3.2334 mL | 6.4668 mL | 8.0836 mL |
| 50 mM | 0.0647 mL | 0.3233 mL | 0.6467 mL | 1.2934 mL | 1.6167 mL |
| 100 mM | 0.0323 mL | 0.1617 mL | 0.3233 mL | 0.6467 mL | 0.8084 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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Synthesis and characterization of 4-methoxy-7-nitroindolinyl-D-aspartate, a caged compound for selective activation of glutamate transporters and N-methyl-D-aspartate receptors in brain tissue.[Pubmed:15736942]
Biochemistry. 2005 Mar 8;44(9):3316-26.
The D-isomer of aspartate is efficiently transported by high-affinity Na(+)/K(+)-dependent glutamate transporters and is an effective ligand of N-methyl-d-aspartate (NMDA) receptors. To facilitate analysis of the regulation of these proteins in their native membranes, we synthesized a photolabile analogue of D-aspartate, 4-methoxy-7-nitroindolinyl-D-aspartate (MNI-D-aspartate). This compound was photolyzed with a quantum efficiency of 0.09 at pH 7.4. Photorelease of d-aspartate in acute hippocampal slices through brief (1 ms) UV laser illumination of MNI-d-aspartate triggered rapidly activating currents in astrocytes that were inhibited by the glutamate transporter antagonist DL-threo-beta-benzyloxyaspartic acid (TBOA), indicating that they resulted from electrogenic uptake of D-aspartate. These transporter currents exhibited a distinct tail component that was approximately 2% of the peak current, which may result from the release of K(+) into the extracellular space during counter transport. MNI-D-aspartate was neither an agonist nor an antagonist of glutamate transporters at concentrations up to 500 muM and was stable in aqueous solution for several days. Glutamate transporter currents were also elicited in Bergmann glial cells and Purkinje neurons of the cerebellum in response to photolysis of MNI-D-aspartate, indicating that this compound can be used for monitoring the occupancy and regulation of glutamate transporters in different brain regions. Photorelease of D-aspartate did not activate alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate receptors or metabotropic glutamate receptors (mGluRs) in neurons, but resulted in the selective, but transient, activation of NMDA receptors in hippocampal pyramidal neurons; MNI-D-aspartate was not an antagonist of NMDA receptors. These results indicate that MNI-D-aspartate also may be useful for studying the regulation of NMDA receptors at excitatory synapses.
