Maackiain

CAS# 19908-48-6

Maackiain

Catalog No. BCN1236----Order now to get a substantial discount!

Product Name & Size Price Stock
Maackiain: 5mg $92 In Stock
Maackiain: 10mg Please Inquire In Stock
Maackiain: 20mg Please Inquire Please Inquire
Maackiain: 50mg Please Inquire Please Inquire
Maackiain: 100mg Please Inquire Please Inquire
Maackiain: 200mg Please Inquire Please Inquire
Maackiain: 500mg Please Inquire Please Inquire
Maackiain: 1000mg Please Inquire Please Inquire
Related Products

Quality Control of Maackiain

Number of papers citing our products

Chemical structure

Maackiain

3D structure

Chemical Properties of Maackiain

Cas No. 19908-48-6 SDF Download SDF
PubChem ID 161298 Appearance Powder
Formula C16H12O5 M.Wt 284.27
Type of Compound Flavonoids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
SMILES C1C2C(C3=C(O1)C=C(C=C3)O)OC4=CC5=C(C=C24)OCO5
Standard InChIKey HUKSJTUUSUGIDC-BDJLRTHQSA-N
Standard InChI InChI=1S/C16H12O5/c17-8-1-2-9-12(3-8)18-6-11-10-4-14-15(20-7-19-14)5-13(10)21-16(9)11/h1-5,11,16-17H,6-7H2/t11-,16-/m1/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Maackiain

The flowers of Maackia amurensis Rupr.et Maxim.

Biological Activity of Maackiain

DescriptionMaackiain is an antimicrobial compound (phytoalexins), it has anticancer effects, it induces apoptosis and growth suppression. Maackiain also shows strong larvicidal activity (LC50 = 21.95 ± 1.34 ug/mL).
TargetsAntifection
In vitro

Extract of Bowdichia virgilioides and maackiain as larvicidal agent against Aedes aegypti mosquito.[Pubmed: 25819294]

Exp Parasitol. 2015 Jun;153:160-4.


METHODS AND RESULTS:
The larvicidal activities of extracts of three hardwood species (Hymenaea stigonorcapa, Anadenanthera colubrina and Bowdichia virgilioides) against 4th instar larvae of Aedes aegypti were evaluated using WHO guidelines. Extracts of H. stignocarpa and A. colubrina showed weak activity. The highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of B. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/mL. Fraction toluene/EtOAc (8:2) from this extract showed larvicidal activity (LC₅₀ = 34.90 ± 1.27 µg/mL). A mixture of two compounds identified as medicarpin and Maackiain exhibited a very good larvicidal activity (sub-fraction 2, LC₅₀ = 17.5 ± 1.87 µg/mL) and Maackiain showed to be a strong larvicidal compound (LC₅₀ = 21.95 ± 1.34 µg/mL).
CONCLUSIONS:
This result can be of value in the search for new natural larvicidal compounds from other hardwood plant extracts and presents the first report of B. virgilioides being used to control a mosquito vector.

Induction of apoptosis by maackiain and trifolirhizin (maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) in human promyelotic leukemia HL-60 cells.[Pubmed: 15547735]

Oncol Rep. 2004 Dec;12(6):1183-8.


METHODS AND RESULTS:
We have investigated the effects of Maackiain and trifolirhizin (Maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) on DNA of human promyelotic HL-60 leukemia cells. It was found that extent of induction of apoptosis by Maackiain was larger than that by trifolirhizin in human leukemia HL-60 cells. Morphological changes showing apoptotic bodies were observed in the HL-60 cells treated with Maackiain and trifolirhizin. The fragmentations of DNA by Maackiain and trifolirhizin to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in the HL-60 cells.
CONCLUSIONS:
The data of the present study show that the suppressions by Maackiain and trifolrhizin of growth of the HL-60 cells result from the induction of apoptosis by these compounds, and that the extent of growth suppression and induction of apoptosis by Maackiain was greater than that by the glycoside (trifolirhizin).

Protocol of Maackiain

Kinase Assay

Three Genes for Metabolism of the Phytoalexin Maackiain in the Plant Pathogen Nectria haematococca: Meiotic Instability and Relationship to a New Gene for Pisatin Demethylase.[Pubmed: 16348671 ]

Appl Environ Microbiol. 1992 Mar;58(3):801-8.

Some isolates of the plant-pathogenic fungus Nectria haematococca mating population (MP) VI metabolize Maackiain and medicarpin, two antimicrobial compounds (phytoalexins) synthesized by chickpea (Cicer arietinum L.). The enzymatic modifications by the fungus convert the phytoalexins to less toxic derivatives, and this detoxification has been proposed to be important for pathogenesis on chickpea.
METHODS AND RESULTS:
In the present study, loci controlling Maackiain metabolism (Mak genes) were identified by crosses among isolates of N. haematococca MP VI that differed in their ability to metabolize the phytoalexin. Strains carrying Mak1 or Mak2 converted Maackiain to 1a-hydroxyMaackiain, while those with Mak3 converted it to 6a-hydroxyMaackiain. Mak1 and Mak2 were unusual in that they often failed to be inherited by progeny. Mak1 was closely linked to Pda6, a new member in a family of genes in N. haematococca MP VI that encode enzymes for detoxification of pisatin, the phytoalexin synthesized by garden pea. Like Mak1, Pda6 was also transmitted irregularly to progeny.
CONCLUSIONS:
Although the unusual meiotic behaviors of some Mak genes complicate genetic analysis, identification of these genes should afford a more through evaluation of the role of phytoalexin detoxification in the pathogenesis of N. haematococca MP VI on chickpea.

Maackiain Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Maackiain Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of Maackiain

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.5178 mL 17.5889 mL 35.1778 mL 70.3556 mL 87.9446 mL
5 mM 0.7036 mL 3.5178 mL 7.0356 mL 14.0711 mL 17.5889 mL
10 mM 0.3518 mL 1.7589 mL 3.5178 mL 7.0356 mL 8.7945 mL
50 mM 0.0704 mL 0.3518 mL 0.7036 mL 1.4071 mL 1.7589 mL
100 mM 0.0352 mL 0.1759 mL 0.3518 mL 0.7036 mL 0.8794 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University
Featured Products
New Products
 

References on Maackiain

A gene for maackiain detoxification from a dispensable chromosome of Nectria haematococca.[Pubmed:8709942]

Mol Gen Genet. 1996 Jun 24;251(4):397-406.

In Nectria haematococca the MAK1 gene product converts a chick-pea (Cicer arietinum) phytoalexin, Maackiain, into a less toxic compound. The presence of MAK1 in this fungal pathogen is also correlated with high virulence on chick-pea. Previous genetic analysis suggested that MAK1 is located on a meiotically unstable, dispensable chromosome. The unstable nature of this chromosome facilitated MAK1 cloning by allowing us to identify a subset of genomic cosmid clones likely to contain MAK1. Truncated forms of the chromosome, generated during meiosis, were isolated from strains either able (Mak+) or unable (Mak-) to metabolize Maackiain and used to probe a chromosome-specific cosmid library. Only clones that hybridized exclusively to the chromosome from the Mak+ strain were then screened for their ability to transform a Mak- isolate to the Mak+ phenotype. A 2.7 kb HindIII-PstI fragment was subcloned from a cosmid conferring MAK1 activity, and its nucleotide sequence determined. Because MAK1 transcription is not induced strongly by Maackiain, a reverse transcriptase-polymerase chain reaction was required to detect MAK1 transcription in a Mak+ strain, and to isolate MAK1 cDNA fragments. Comparison of the genomic and cDNA sequences of MAK1 revealed the presence of three introns and an open reading frame encoding a protein 460 amino acids in length. Two diagnostic domains in its deduced amino acid sequence suggest MAK1 encodes a flavin-containing mono-oxygenase. MAK1 is the first gene encoding Maackiain detoxification to be cloned, and is the second functional gene cloned from this dispensable chromosome. Southern analysis of genomic DNA from ascospore isolates containing MAK2, MAK3, and MAK4 indicated that MAK1 is not homologous to other known maackianin-detoxifying genes.

Three Genes for Metabolism of the Phytoalexin Maackiain in the Plant Pathogen Nectria haematococca: Meiotic Instability and Relationship to a New Gene for Pisatin Demethylase.[Pubmed:16348671]

Appl Environ Microbiol. 1992 Mar;58(3):801-8.

Some isolates of the plant-pathogenic fungus Nectria haematococca mating population (MP) VI metabolize Maackiain and medicarpin, two antimicrobial compounds (phytoalexins) synthesized by chickpea (Cicer arietinum L.). The enzymatic modifications by the fungus convert the phytoalexins to less toxic derivatives, and this detoxification has been proposed to be important for pathogenesis on chickpea. In the present study, loci controlling Maackiain metabolism (Mak genes) were identified by crosses among isolates of N. haematococca MP VI that differed in their ability to metabolize the phytoalexin. Strains carrying Mak1 or Mak2 converted Maackiain to 1a-hydroxyMaackiain, while those with Mak3 converted it to 6a-hydroxyMaackiain. Mak1 and Mak2 were unusual in that they often failed to be inherited by progeny. Mak1 was closely linked to Pda6, a new member in a family of genes in N. haematococca MP VI that encode enzymes for detoxification of pisatin, the phytoalexin synthesized by garden pea. Like Mak1, Pda6 was also transmitted irregularly to progeny. Although the unusual meiotic behaviors of some Mak genes complicate genetic analysis, identification of these genes should afford a more through evaluation of the role of phytoalexin detoxification in the pathogenesis of N. haematococca MP VI on chickpea.

Induction of apoptosis by maackiain and trifolirhizin (maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) in human promyelotic leukemia HL-60 cells.[Pubmed:15547735]

Oncol Rep. 2004 Dec;12(6):1183-8.

We have investigated the effects of Maackiain and trifolirhizin (Maackiain glycoside) isolated from sanzukon (Sophora Subprostrate Chen et T. Chen) on DNA of human promyelotic HL-60 leukemia cells. It was found that extent of induction of apoptosis by Maackiain was larger than that by trifolirhizin in human leukemia HL-60 cells. Morphological changes showing apoptotic bodies were observed in the HL-60 cells treated with Maackiain and trifolirhizin. The fragmentations of DNA by Maackiain and trifolirhizin to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in the HL-60 cells. The data of the present study show that the suppressions by Maackiain and trifolrhizin of growth of the HL-60 cells result from the induction of apoptosis by these compounds, and that the extent of growth suppression and induction of apoptosis by Maackiain was greater than that by the glycoside (trifolirhizin).

Extract of Bowdichia virgilioides and maackiain as larvicidal agent against Aedes aegypti mosquito.[Pubmed:25819294]

Exp Parasitol. 2015 Jun;153:160-4.

The larvicidal activities of extracts of three hardwood species (Hymenaea stigonorcapa, Anadenanthera colubrina and Bowdichia virgilioides) against 4th instar larvae of Aedes aegypti were evaluated using WHO guidelines. Extracts of H. stignocarpa and A. colubrina showed weak activity. The highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of B. virgilioides, which caused 100% mortality at concentrations at 50 and 100 microg/mL. Fraction toluene/EtOAc (8:2) from this extract showed larvicidal activity (LC(5)(0) = 34.90 +/- 1.27 microg/mL). A mixture of two compounds identified as medicarpin and Maackiain exhibited a very good larvicidal activity (sub-fraction 2, LC(5)(0) = 17.5 +/- 1.87 microg/mL) and Maackiain showed to be a strong larvicidal compound (LC(5)(0) = 21.95 +/- 1.34 microg/mL). This result can be of value in the search for new natural larvicidal compounds from other hardwood plant extracts and presents the first report of B. virgilioides being used to control a mosquito vector.

Description

Maackiain (DL-Maackiain) is isolated from Maackia amurensis Rupr.et Maxim. Maackiain (DL-Maackiain) is a larvicidal agent against Aedes aegypti mosquito.xp Parasitol with a LD50 of 21.95 µg/mL. Maackiain (DL-Maackiain) induces fragmentations of DNA to oligonucleosomal-sized fragments that like a characteristic of apoptosis in the HL-60 cells.

Keywords:

Maackiain,19908-48-6,Natural Products, buy Maackiain , Maackiain supplier , purchase Maackiain , Maackiain cost , Maackiain manufacturer , order Maackiain , high purity Maackiain

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: