NeotuberostemonineCAS# 143120-46-1 |
- Tuberstemonine
Catalog No.:BCN4986
CAS No.:6879-01-2
Quality Control & MSDS
Number of papers citing our products
Chemical structure
3D structure
Cas No. | 143120-46-1 | SDF | Download SDF |
PubChem ID | 11667940 | Appearance | Powder |
Formula | C22H33NO4 | M.Wt | 375.5 |
Type of Compound | Alkaloids | Storage | Desiccate at -20°C |
Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
SMILES | CCC1C2CCCCN3C2C(CC3C4CC(C(=O)O4)C)C5C1OC(=O)C5C | ||
Standard InChIKey | GYOGHROCTSEKDY-UEIGSNQUSA-N | ||
Standard InChI | InChI=1S/C22H33NO4/c1-4-13-14-7-5-6-8-23-16(17-9-11(2)21(24)26-17)10-15(19(14)23)18-12(3)22(25)27-20(13)18/h11-20H,4-10H2,1-3H3/t11-,12-,13+,14+,15-,16-,17-,18-,19+,20+/m0/s1 | ||
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. |
Description | 1. Neotuberostemonine (NTS) is one of the main antitussive alkaloids in the root of Stemona tuberosa Lour, it has a significant protective effect on bleomycin (BLM)-induced pulmonary fibrosis through suppressing the recruitment and M2 polarization of macrophages. 2. Neotuberostemonine demonstrates antitussive properties in guinea pigs. |
Targets | TGF-β/Smad | MMP(e.g.TIMP) | NOS |
Neotuberostemonine Dilution Calculator
Neotuberostemonine Molarity Calculator
1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
1 mM | 2.6631 mL | 13.3156 mL | 26.6312 mL | 53.2623 mL | 66.5779 mL |
5 mM | 0.5326 mL | 2.6631 mL | 5.3262 mL | 10.6525 mL | 13.3156 mL |
10 mM | 0.2663 mL | 1.3316 mL | 2.6631 mL | 5.3262 mL | 6.6578 mL |
50 mM | 0.0533 mL | 0.2663 mL | 0.5326 mL | 1.0652 mL | 1.3316 mL |
100 mM | 0.0266 mL | 0.1332 mL | 0.2663 mL | 0.5326 mL | 0.6658 mL |
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. |
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
- BQ-3020
Catalog No.:BCC5728
CAS No.:143113-45-5
- Phytic acid sodium salt hydrate
Catalog No.:BCN1283
CAS No.:14306-25-3
- Ethyl 3,4-dicaffeoylquinate
Catalog No.:BCN8004
CAS No.:143051-73-4
- 1alpha-Hydroxy VD4
Catalog No.:BCC1300
CAS No.:143032-85-3
- ML216
Catalog No.:BCC8061
CAS No.:1430213-30-1
- Vinblastine Sulfate
Catalog No.:BCN2292
CAS No.:143-67-9
- Protoveratrine A
Catalog No.:BCN5346
CAS No.:143-57-7
- Lauric acid
Catalog No.:BCN2635
CAS No.:143-07-7
- Salvianolic acid D
Catalog No.:BCN2369
CAS No.:142998-47-8
- Salvianolic acid E
Catalog No.:BCN8194
CAS No.:142998-46-7
- Fmoc-D-Ala(3-pyridyl)-OH.HCl
Catalog No.:BCC3324
CAS No.:142994-45-4
- Fmoc-D-Phe(4-Cl)-OH
Catalog No.:BCC3177
CAS No.:142994-19-2
- GSK-LSD1 2HCl
Catalog No.:BCC5647
CAS No.:1431368-48-7
- BMX-IN-1
Catalog No.:BCC1434
CAS No.:1431525-23-3
- H-D-Ala-OMe.HCl
Catalog No.:BCC3199
CAS No.:14316-06-4
- UNC1999
Catalog No.:BCC4552
CAS No.:1431612-23-5
- CAL-130
Catalog No.:BCC1440
CAS No.:1431697-74-3
- CAL-130 Hydrochloride
Catalog No.:BCC1441
CAS No.:1431697-78-7
- gamma-secretase modulator 3
Catalog No.:BCC1585
CAS No.:1431697-84-5
- AT7519 trifluoroacetate
Catalog No.:BCC1377
CAS No.:1431697-85-6
- AT7867 dihydrochloride
Catalog No.:BCC1378
CAS No.:1431697-86-7
- OTSSP167
Catalog No.:BCC4314
CAS No.:1431697-89-0
- SB-408124 Hydrochloride
Catalog No.:BCC1929
CAS No.:1431697-90-3
- CCT241533 hydrochloride
Catalog No.:BCC1463
CAS No.:1431697-96-9
Antitussive and central respiratory depressant effects of Stemona tuberosa.[Pubmed:20219659]
J Ethnopharmacol. 2010 Apr 21;128(3):679-84.
AIMS OF THE STUDY: Stemona alkaloids with distinctly different chemical skeletons are recently reported as the active components in the antitussive herb Baibu derived from the root-tubers of Stemona tuberosa. This study aims to determine if alkaloids of this herb contribute equally to the antitussive functions, act on the same sites of cough reflex, and play any role in inducing central respiratory depressant effects. MATERIALS AND METHODS: Antitussive potency of four major alkaloids was evaluated on guinea pigs with citric acid aerosol to induce cough. The action sites of the alkaloids on cough reflex pathway were tested with electrical stimulation of the superior laryngeal nerve in guinea pigs. The central respiratory effects of croomine were also tested on guinea pigs. RESULTS: Croomine, Neotuberostemonine and stemoninine showed similar antitussive potency, while tuberostemonine showed much weaker antitussive potency. Neotuberostemonine, tuberostemonine and stemoninine acted on the peripheral cough reflex pathway, while croomine acted on the central part. Croomine also showed obvious central respiratory depressant effects. CONCLUSIONS: The four major Stemona alkaloids in Stemona tuberosa do not contribute equally to antitussive potency in guinea pigs. Neotuberostemonine, tuberostemonine and stemoninine target on peripheral cough reflex pathway. Croomine acts on central sites in the cough reflex pathway and demonstrates central respiratory depressant effects, which can partly account for the adverse reactions reported for the herb.
Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages.[Pubmed:27144994]
Int Immunopharmacol. 2016 Jul;36:158-164.
Neotuberostemonine (NTS) is one of the main antitussive alkaloids in the root of Stemona tuberosa Lour. This study aimed to investigate the effects of NTS on bleomycin (BLM)-induced pulmonary fibrosis in mice and the underlying mechanism. After BLM administration, NTS were orally administered to mice at 20 and 40mg/kg per day from days 8 to 21, with nintedanib as a positive control. The effect of NTS on BLM-induced mice was assessed via histopathological examination by HE and Masson's trichrome staining, TGF-beta1 level and macrophage recruitment by immunohistochemical staining, expression of profibrotic media and M1/M2 polarization by western blot. RAW 264.7 cells were used to evaluate whether NTS (1, 10, 100muM) directly affected macrophages. The results revealed that NTS treatment significantly ameliorated lung histopathological changes and decreased inflammatory cell counts in the bronchoalveolar lavage fluid. The over-expression of collagen, alpha-SMA and TGF-beta1 was reduced by NTS. Furthermore, NTS markedly lowered the expression of MMP-2 and TIMP-1 while raised the expression of MMP-9. A further analysis showed that NTS was able to decrease the recruitment of macrophages and to inhibit the M2 polarization in mice lung tissues. The experiment in vitro showed that NTS significantly reduced the arginase-1 (marker for M2) expression in a dose-dependent manner but down-regulated the iNOS (marker for M1) expression only at 100muM. In conclusion, our study demonstrated for the first time that NTS has a significant protective effect on BLM-induced pulmonary fibrosis through suppressing the recruitment and M2 polarization of macrophages.
TLC-image analysis of non-chromophoric tuberostemonine alkaloid derivatives in Stemona species.[Pubmed:24079167]
Nat Prod Commun. 2013 Aug;8(8):1065-8.
A simple, selective, precise, and accurate thin-layer chromatographic (TLC) image analytical method was developed and validated for simultaneous quantification of the major components in the root extracts of Stemona tuberosa (tuberostemonine, tuberostemonine N and Neotuberostemonine)), and S. phyllantha (tuberostemonine and tuberostemonine A). The analysis was performed by TLC on silica gel 60 F254 aluminum plates using a mixture of dichloromethane: ethyl acetate: methanol: ammonium hydroxide (50:45:4:1) as mobile phase. Post-derivatization was employed by dipping the TLC plate into Dragendorff's reagent to visualize the spots. Image analysis of the scanned TLC plate was performed to detect the contents of tuberostemonine derivatives. The polynomial regression data for the calibration plots showed good linear relationships within the concentration range of 2-7 microg/spot. The method gave satisfactory precision, accuracy, selectivity and could simultaneously quantify tuberostemonine, tuberostemonine A, tuberostemonine N and Neotuberostemonine. Dried powdered roots of S. tuberosa grown in Thailand contained 1.31 +/- 0.28, 1.63 +/- 0.18 and 1.24 +/- 0.27% tuberostemonine, tuberostemonine N, and Neotuberostemonine (dry weight), respectively, while S. phyllantha roots contained 1.39 +/- 0.14% tuberostemonine and 0.39 +/- 0.08% tuberostemonine A (dry weight). The proposed method was simple, inexpensive, and more accessible to apply for many local authorities and small laboratories.