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3-Methylcatechol

CAS# 488-17-5

3-Methylcatechol

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Quality Control of 3-Methylcatechol

Number of papers citing our products

Chemical structure

3-Methylcatechol

3D structure

Chemical Properties of 3-Methylcatechol

Cas No. 488-17-5 SDF Download SDF
PubChem ID 340 Appearance Oil
Formula C7H8O2 M.Wt 124.1
Type of Compound Phenols Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 3-methylbenzene-1,2-diol
SMILES CC1=C(C(=CC=C1)O)O
Standard InChIKey PGSWEKYNAOWQDF-UHFFFAOYSA-N
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of 3-Methylcatechol

The Phellinus igniarius

Biological Activity of 3-Methylcatechol

TargetsCalcium Channel

3-Methylcatechol Dilution Calculator

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3-Methylcatechol Molarity Calculator

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Preparing Stock Solutions of 3-Methylcatechol

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 8.058 mL 40.2901 mL 80.5802 mL 161.1604 mL 201.4504 mL
5 mM 1.6116 mL 8.058 mL 16.116 mL 32.2321 mL 40.2901 mL
10 mM 0.8058 mL 4.029 mL 8.058 mL 16.116 mL 20.145 mL
50 mM 0.1612 mL 0.8058 mL 1.6116 mL 3.2232 mL 4.029 mL
100 mM 0.0806 mL 0.4029 mL 0.8058 mL 1.6116 mL 2.0145 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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References on 3-Methylcatechol

A process optimization for bio-catalytic production of substituted catechols (3-nitrocatechol and 3-methylcatechol.[Pubmed:20587073]

BMC Biotechnol. 2010 Jun 30;10:49.

BACKGROUND: Substituted catechols are important precursors for large-scale synthesis of pharmaceuticals and other industrial products. Most of the reported chemical synthesis methods are expensive and insufficient at industrial level. However, biological processes for production of substituted catechols could be highly selective and suitable for industrial purposes. RESULTS: We have optimized a process for bio-catalytic production of 3-substituted catechols viz. 3-nitrocatechol (3-NC) and 3-Methylcatechol (3-MC) at pilot scale. Amongst the screened strains, two strains viz. Pseudomonas putida strain (F1) and recombinant Escherichia coli expression clone (pDTG602) harboring first two genes of toluene degradation pathway were found to accumulate 3-NC and 3-MC respectively. Various parameters such as amount of nutrients, pH, temperature, substrate concentration, aeration, inoculums size, culture volume, toxicity of substrate and product, down stream extraction, single step and two-step biotransformation were optimized at laboratory scale to obtain high yields of 3-substituted catechols. Subsequently, pilot scale studies were performed in 2.5 liter bioreactor. The rate of product accumulation at pilot scale significantly increased up to approximately 90-95% with time and high yields of 3-NC (10 mM) and 3-MC (12 mM) were obtained. CONCLUSION: The biocatalytic production of 3-substituted catechols viz. 3-NC and 3-MC depend on some crucial parameters to obtain maximum yields of the product at pilot scale. The process optimized for production of 3-substituted catechols by using the organisms P. putida (F1) and recombinant E. coli expression clone (pDTG602) may be useful for industrial application.

Enhanced 3-methylcatechol production by Pseudomonas putida TODE1 in a two-phase biotransformation system.[Pubmed:25420423]

J Gen Appl Microbiol. 2014;60(5):183-90.

In this study, genetically engineered Pseudomonas putida TODE1 served as a biocatalyst for the bioproduction of valuable 3-Methylcatechol (3MC) from toluene in an aqueous-organic two-phase system. The two-phase system was used as an approach to increase the biocatalyst efficiency. Among the organic solvent tested, n-decanol offered several benefits including having the highest partitioning of 3MC, with a high 3MC yield and low cell toxicity. The effect of media supplementation with carbon/energy sources (glucose, glycerol, acetate and succinate), divalent metal cations (Mg(2+), Ca(2+), Mn(2+) and Fe(2+)), and short-chain alcohols (ethanol, n-propanol and n-butanol) as a cofactor regeneration system on the toluene dioxygenase (TDO) activity, cell viability, and overall 3MC yield were evaluated. Along with the two-step cell preparation protocol, supplementation of the medium with 4 mM glycerol as a carbon/energy source, and 0.4 mM Fe(2+) as a cofactor for TDO significantly enhanced the 3MC production level. When in combination with the use of n-decanol and n-butanol as the organic phase, a maximum overall 3MC concentration of 31.8 mM (166 mM in the organic phase) was obtained in a small-scale production, while it was at 160.5 mM (333.2 mM in the organic phase) in a 2-L scale. To our knowledge, this is the highest 3MC yield obtained from a TDO-based system so far.

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