Phillyrin

CAS# 487-41-2

Phillyrin

Catalog No. BCN1096----Order now to get a substantial discount!

Product Name & Size Price Stock
Phillyrin: 5mg $12 In Stock
Phillyrin: 10mg Please Inquire In Stock
Phillyrin: 20mg Please Inquire Please Inquire
Phillyrin: 50mg Please Inquire Please Inquire
Phillyrin: 100mg Please Inquire Please Inquire
Phillyrin: 200mg Please Inquire Please Inquire
Phillyrin: 500mg Please Inquire Please Inquire
Phillyrin: 1000mg Please Inquire Please Inquire

Quality Control of Phillyrin

Number of papers citing our products

Chemical structure

Phillyrin

3D structure

Chemical Properties of Phillyrin

Cas No. 487-41-2 SDF Download SDF
PubChem ID 134692163 Appearance Powder
Formula C27H34O11 M.Wt 534.56
Type of Compound Lignans Storage Desiccate at -20°C
Synonyms Forsythin; Phillyroside; Phyllyrin
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name (2S,5S)-2-[4-[(3R,3aR,6S,6aR)-3-(3,4-dimethoxyphenyl)-1,3,3a,4,6,6a-hexahydrofuro[3,4-c]furan-6-yl]-3-methoxyphenoxy]-6-(hydroxymethyl)oxane-3,4,5-triol
SMILES COC1=C(C=C(C=C1)C2C3COC(C3CO2)C4=C(C=C(C=C4)OC5C(C(C(C(O5)CO)O)O)O)OC)OC
Standard InChIKey ZTIZEXUIEBLYBL-MNXUFRCLSA-N
Standard InChI InChI=1S/C27H34O11/c1-32-18-7-4-13(8-20(18)34-3)25-16-11-36-26(17(16)12-35-25)15-6-5-14(9-19(15)33-2)37-27-24(31)23(30)22(29)21(10-28)38-27/h4-9,16-17,21-31H,10-12H2,1-3H3/t16-,17-,21?,22+,23?,24?,25-,26+,27+/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Phillyrin

1 Forsythia sp.

Biological Activity of Phillyrin

DescriptionPhillyrin is a novel AMPK activator, has anti-obesity effects in nutritive obesity mice, it can prevent lipid accumulation in HepG2 cells by blocking the expression of SREBP-1c and FAS through LKB1/AMPK activation. Phillyrin may be a new preventive agent of acute lung injury in the clinical setting, it potentially contributes to the suppression of the activation of MAPK and NF-κB pathways, it also has protective effects on H2O2-induced oxidative stress and apoptosis in PC12 cells.
TargetsFatty Acid Synthase | AMPK | ROS | Bcl-2/Bax | TNF-α | IL Receptor | MAPK | NF-kB
In vitro

Phillyrin attenuates high glucose-induced lipid accumulation in human HepG2 hepatocytes through the activation of LKB1/AMP-activated protein kinase-dependent signalling.[Pubmed: 23122079]

Food Chem. 2013 Jan 15;136(2):415-25.

Phillyrin, an active constituent found in many medicinal plants and certain functional foods, has anti-obesity activity in vivo. The aim of our study was to provide new data on the molecular mechanism(s) underlying the role of Phillyrin in the prevention of high glucose-induced lipid accumulation in human HepG2 hepatocytes.
METHODS AND RESULTS:
We found that Phillyrin suppressed high glucose-induced lipid accumulation in HepG2 cells. Phillyrin strongly inhibited high glucose-induced fatty acid synthase (FAS) expression by modulating sterol regulatory element-binding protein-1c (SREBP-1c) activation. Moreover, use of the pharmacological AMP-activated protein kinase (AMPK) inhibitor compound C revealed that AMPK is essential for suppressing SREBP-1c expression in Phillyrin-treated cells. Finally, we found that liver kinase B1 (LKB1) phosphorylation is required for the Phillyrin-enhanced activation of AMPK in HepG2 hepatocytes.
CONCLUSIONS:
These results indicate that Phillyrin prevents lipid accumulation in HepG2 cells by blocking the expression of SREBP-1c and FAS through LKB1/AMPK activation, suggesting that Phillyrin is a novel AMPK activator with a role in the prevention and treatment of obesity.

In vivo

[Effect of phillyrin on the anti-obesity in nutritive obesity mice].[Pubmed: 15981888]

Zhong Yao Cai. 2005 Feb;28(2):123-4.

To study on the anti-obesity effect of Phillyrin in nutritive obesity mice.
METHODS AND RESULTS:
The alimentary obesity model was established by hyperalimentation. The wet weight of fat, fat index, number of fat cells per unit visual field, Lee's index, jejunum microvillus area, serum triglyceride and cholesterol were selected to observe the anti-obesity effect of Phillyrin. Phillyrin could lower wet weight of fat (P < 0.01), fat index (P < 0.05 or P < 0.01), diameter of fat cell and Lee's index (P < 0.05), decrease the jejunum microvillus area, lower the level of serum triglyceride and cholesterol.
CONCLUSIONS:
Phillyrin has anti-obesity effect in nutritive obesity mice.

Protocol of Phillyrin

Cell Research

Protective effects of phillyrin on H2O 2-induced oxidative stress and apoptosis in PC12 cells.[Pubmed: 25146667]

Cell Mol Neurobiol. 2014 Nov;34(8):1165-73.

Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. We sought to elucidate possible effects of Phillyrin, an active constituent isolated from the Chinese medicinal herb Forsythia suspense, on hydrogen peroxide (H2O2)-induced cell death and determine the underlying molecular mechanisms in neuron-like PC12 cells.
METHODS AND RESULTS:
By MTT assay and lactate dehydrogenase (LDH) leakage assay, we found that Phillyrin treatment effectively protected PC12 cells against H2O2-induced cell damage. H2O2 exposure induced oxidative stress in PC12 cells, as revealed by enhanced oxidative stress and decreased activities of antioxidative enzymes, which were inhibited by Phillyrin pretreatment. ROS activated mitochondria-dependent apoptosis. The anti-apoptotic effects of Phillyrin were also confirmed by acridine orange/ethidium bromide (AO/EB) staining. Mitochondrial membrane potential decrease, cytochrome c release, caspases activation, activation of AIF and Endo G were observed in H2O2-treated cells by rhodamine 123 or western blot. Interestingly, Phillyrin effectively suppressed these changes. Moreover, Phillyrin could inhibit H2O2-induced up-regulation of Bax/Bcl-2 ratio.
CONCLUSIONS:
In conclusion, Phillyrin effectively inhibited H2O2-induced oxidative stress and apoptosis in PC12 cells.

Animal Research

Phillyrin attenuates LPS-induced pulmonary inflammation via suppression of MAPK and NF-κB activation in acute lung injury mice.[Pubmed: 23751215]

Pharmacokinetics of phillyrin and forsythiaside following iv administration to Beagle dog.[Pubmed: 19645216]

Eur J Drug Metab Pharmacokinet. 2009 Apr-Jun;34(2):79-83.

The objective of the present study was to firstly investigate the in vivo pharmacokinetics of Phillyrin and forsythiaside in beagle dog.
METHODS AND RESULTS:
On I.V. administration, a rapid distribution was observed and followed by a slower elimination for Phillyrin and forsythiaside. The mean t(1/2Z) was 49.99, 34.87 and 43.81 min for 0.19, 0.70 and 1.43 mg/kg of Phillyrin, and 60.90, 64.30, 57.99 min for 0.62, 1.39 and 5.52 mg/kg of forsythiaside respectively. And the AUC(o-t) increased linearly from 36.51 to 160.22 microg x min/ml of Phillyrin and from 50.63 to 681.08 microg x min/ml after the three dosage administrated. In the range of the dose examined, the pharmacokinetics of Phillyrin and forsythiaside in beagle dog was based on first order kinetics.
CONCLUSIONS:
Although both drugs were widely distributed to various tissues in the dog, no concerns about extensive binding to tissues that may be consumed by the public should a dog be exposed to Phillyrin and forsythiaside according to the rapid elimination.

Fitoterapia. 2013 Oct;90:132-9.

Phillyrin (Phil) is one of the main chemical constituents of Forsythia suspensa (Thunb.), which has shown to be an important traditional Chinese medicine. We tested the hypothesis that Phil modulates pulmonary inflammation in an ALI model induced by LPS.
METHODS AND RESULTS:
Male BALB/c mice were pretreated with or without Phil before respiratory administration with LPS, and pretreated with dexamethasone as a control. Cytokine release (TNF-α, IL-1β, and IL-6) and amounts of inflammatory cell in bronchoalveolar lavage fluid (BALF) were detected by ELISA and cell counting separately. Pathologic changes, including neutrophil infiltration, interstitial edema, hemorrhage, hyaline membrane formation, necrosis, and congestion during acute lung injury in mice were evaluated via pathological section with HE staining. To further investigate the mechanism of Phil anti-inflammatory effects, activation of MAPK and NF-κB pathways was tested by western blot assay. Phil pretreatment significantly attenuated LPS-induced pulmonary histopathologic changes, alveolar hemorrhage, and neutrophil infiltration. The lung wet-to-dry weight ratios, as the index of pulmonary edema, were markedly decreased by Phil pretreatment. In addition, Phil decreased the production of the proinflammatory cytokines including (TNF-α, IL-1β, and IL-6) and the concentration of myeloperoxidase (MPO) in lung tissues. Phil pretreatment also significantly suppressed LPS-induced activation of MAPK and NF-κB pathways in lung tissues.
CONCLUSIONS:
Taken together, the results suggest that Phil may have a protective effect on LPS-induced ALI, and it potentially contributes to the suppression of the activation of MAPK and NF-κB pathways. Phil may be a new preventive agent of ALI in the clinical setting.

Phillyrin Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Phillyrin Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of Phillyrin

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.8707 mL 9.3535 mL 18.707 mL 37.4139 mL 46.7674 mL
5 mM 0.3741 mL 1.8707 mL 3.7414 mL 7.4828 mL 9.3535 mL
10 mM 0.1871 mL 0.9353 mL 1.8707 mL 3.7414 mL 4.6767 mL
50 mM 0.0374 mL 0.1871 mL 0.3741 mL 0.7483 mL 0.9353 mL
100 mM 0.0187 mL 0.0935 mL 0.1871 mL 0.3741 mL 0.4677 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University
Featured Products
New Products
 

References on Phillyrin

[Effect of phillyrin on the anti-obesity in nutritive obesity mice].[Pubmed:15981888]

Zhong Yao Cai. 2005 Feb;28(2):123-4.

OBJECTIVE: To study on the anti-obesity effect of Phillyrin in nutritive obesity mice. METHOD: The alimentary obesity model was established by hyperalimentation. The wet weight of fat, fat index, number of fat cells per unit visual field, Lee's index, jejunum microvillus area, serum triglyceride and cholesterol were selected to observe the anti-obesity effect of Phillyrin. RESULT: Phillyrin could lower wet weight of fat (P < 0.01), fat index (P < 0.05 or P < 0.01), diameter of fat cell and Lee's index (P < 0.05), decrease the jejunum microvillus area, lower the level of serum triglyceride and cholesterol. CONCLUSION: Phillyrin has anti-obesity effect in nutritive obesity mice.

Protective effects of phillyrin on H2O 2-induced oxidative stress and apoptosis in PC12 cells.[Pubmed:25146667]

Cell Mol Neurobiol. 2014 Nov;34(8):1165-73.

Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. We sought to elucidate possible effects of Phillyrin, an active constituent isolated from the Chinese medicinal herb Forsythia suspense, on hydrogen peroxide (H2O2)-induced cell death and determine the underlying molecular mechanisms in neuron-like PC12 cells. By MTT assay and lactate dehydrogenase (LDH) leakage assay, we found that Phillyrin treatment effectively protected PC12 cells against H2O2-induced cell damage. H2O2 exposure induced oxidative stress in PC12 cells, as revealed by enhanced oxidative stress and decreased activities of antioxidative enzymes, which were inhibited by Phillyrin pretreatment. ROS activated mitochondria-dependent apoptosis. The anti-apoptotic effects of Phillyrin were also confirmed by acridine orange/ethidium bromide (AO/EB) staining. Mitochondrial membrane potential decrease, cytochrome c release, caspases activation, activation of AIF and Endo G were observed in H2O2-treated cells by rhodamine 123 or western blot. Interestingly, Phillyrin effectively suppressed these changes. Moreover, Phillyrin could inhibit H2O2-induced up-regulation of Bax/Bcl-2 ratio. In conclusion, Phillyrin effectively inhibited H2O2-induced oxidative stress and apoptosis in PC12 cells.

Phillyrin attenuates high glucose-induced lipid accumulation in human HepG2 hepatocytes through the activation of LKB1/AMP-activated protein kinase-dependent signalling.[Pubmed:23122079]

Food Chem. 2013 Jan 15;136(2):415-25.

Phillyrin, an active constituent found in many medicinal plants and certain functional foods, has anti-obesity activity in vivo. The aim of our study was to provide new data on the molecular mechanism(s) underlying the role of Phillyrin in the prevention of high glucose-induced lipid accumulation in human HepG2 hepatocytes. We found that Phillyrin suppressed high glucose-induced lipid accumulation in HepG2 cells. Phillyrin strongly inhibited high glucose-induced fatty acid synthase (FAS) expression by modulating sterol regulatory element-binding protein-1c (SREBP-1c) activation. Moreover, use of the pharmacological AMP-activated protein kinase (AMPK) inhibitor compound C revealed that AMPK is essential for suppressing SREBP-1c expression in Phillyrin-treated cells. Finally, we found that liver kinase B1 (LKB1) phosphorylation is required for the Phillyrin-enhanced activation of AMPK in HepG2 hepatocytes. These results indicate that Phillyrin prevents lipid accumulation in HepG2 cells by blocking the expression of SREBP-1c and FAS through LKB1/AMPK activation, suggesting that Phillyrin is a novel AMPK activator with a role in the prevention and treatment of obesity.

Pharmacokinetics of phillyrin and forsythiaside following iv administration to Beagle dog.[Pubmed:19645216]

Eur J Drug Metab Pharmacokinet. 2009 Apr-Jun;34(2):79-83.

The objective of the present study was to firstly investigate the in vivo pharmacokinetics of Phillyrin and forsythiaside in beagle dog. On I.V. administration, a rapid distribution was observed and followed by a slower elimination for Phillyrin and forsythiaside. The mean t(1/2Z) was 49.99, 34.87 and 43.81 min for 0.19, 0.70 and 1.43 mg/kg of Phillyrin, and 60.90, 64.30, 57.99 min for 0.62, 1.39 and 5.52 mg/kg of forsythiaside respectively. And the AUC(o-t) increased linearly from 36.51 to 160.22 microg x min/ml of Phillyrin and from 50.63 to 681.08 microg x min/ml after the three dosage administrated. In the range of the dose examined, the pharmacokinetics of Phillyrin and forsythiaside in beagle dog was based on first order kinetics. Although both drugs were widely distributed to various tissues in the dog, no concerns about extensive binding to tissues that may be consumed by the public should a dog be exposed to Phillyrin and forsythiaside according to the rapid elimination.

Phillyrin attenuates LPS-induced pulmonary inflammation via suppression of MAPK and NF-kappaB activation in acute lung injury mice.[Pubmed:23751215]

Fitoterapia. 2013 Oct;90:132-9.

Phillyrin (Phil) is one of the main chemical constituents of Forsythia suspensa (Thunb.), which has shown to be an important traditional Chinese medicine. We tested the hypothesis that Phil modulates pulmonary inflammation in an ALI model induced by LPS. Male BALB/c mice were pretreated with or without Phil before respiratory administration with LPS, and pretreated with dexamethasone as a control. Cytokine release (TNF-alpha, IL-1beta, and IL-6) and amounts of inflammatory cell in bronchoalveolar lavage fluid (BALF) were detected by ELISA and cell counting separately. Pathologic changes, including neutrophil infiltration, interstitial edema, hemorrhage, hyaline membrane formation, necrosis, and congestion during acute lung injury in mice were evaluated via pathological section with HE staining. To further investigate the mechanism of Phil anti-inflammatory effects, activation of MAPK and NF-kappaB pathways was tested by western blot assay. Phil pretreatment significantly attenuated LPS-induced pulmonary histopathologic changes, alveolar hemorrhage, and neutrophil infiltration. The lung wet-to-dry weight ratios, as the index of pulmonary edema, were markedly decreased by Phil pretreatment. In addition, Phil decreased the production of the proinflammatory cytokines including (TNF-alpha, IL-1beta, and IL-6) and the concentration of myeloperoxidase (MPO) in lung tissues. Phil pretreatment also significantly suppressed LPS-induced activation of MAPK and NF-kappaB pathways in lung tissues. Taken together, the results suggest that Phil may have a protective effect on LPS-induced ALI, and it potentially contributes to the suppression of the activation of MAPK and NF-kappaB pathways. Phil may be a new preventive agent of ALI in the clinical setting.

Description

Phillyrin is isolated from Forsythia suspensa Vahl (Oleaceae), has antibacterial and anti-inflammatory activities. Phillyrin has potential inductive effects on rat CYP1A2 and CYP2D1 activities, without affecting CYP2C11 and CYP3A1/2 activities. Phillyrin has anti-influenza A virus activities.

Keywords:

Phillyrin,487-41-2,Forsythin; Phillyroside; Phyllyrin,Natural Products, buy Phillyrin , Phillyrin supplier , purchase Phillyrin , Phillyrin cost , Phillyrin manufacturer , order Phillyrin , high purity Phillyrin

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: