Betaine

CAS# 107-43-7

Betaine

Catalog No. BCN6303----Order now to get a substantial discount!

Product Name & Size Price Stock
Betaine: 5mg Please Inquire In Stock
Betaine: 10mg Please Inquire In Stock
Betaine: 20mg Please Inquire Please Inquire
Betaine: 50mg Please Inquire Please Inquire
Betaine: 100mg Please Inquire Please Inquire
Betaine: 200mg Please Inquire Please Inquire
Betaine: 500mg Please Inquire Please Inquire
Betaine: 1000mg Please Inquire Please Inquire

Quality Control of Betaine

Number of papers citing our products

Chemical structure

Betaine

3D structure

Chemical Properties of Betaine

Cas No. 107-43-7 SDF Download SDF
PubChem ID 247 Appearance Powder
Formula C5H11NO2 M.Wt 117.15
Type of Compound Alkaloids Storage Desiccate at -20°C
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name 2-(trimethylazaniumyl)acetate
SMILES C[N+](C)(C)CC(=O)[O-]
Standard InChIKey KWIUHFFTVRNATP-UHFFFAOYSA-N
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Betaine

The roots of Beta vulgaris

Biological Activity of Betaine

DescriptionBetaine protects cells from environmental stress and serves as a methyl donor in several biochemical pathways, it reduces cardiovascular disease risk and protects liver cells from alcoholic liver damage and nonalcoholic steatohepatitis. Betaine has anti-inflammation and tumor preventing capacity, it can inhibit inflammatory cytokines such TNF-α, IL-6, iNOS and COX-2 and decrease the incidence of tumor formation with downregulation of inflammation.
TargetsTNF-α | IL Receptor | NOS | COX | NF-kB
In vitro

Betaine is a positive regulator of mitochondrial respiration.[Pubmed: 25498545]

Biochem Biophys Res Commun. 2015 Jan 9;456(2):621-5.

Betaine protects cells from environmental stress and serves as a methyl donor in several biochemical pathways. It reduces cardiovascular disease risk and protects liver cells from alcoholic liver damage and nonalcoholic steatohepatitis. Its pretreatment can rescue cells exposed to toxins such as rotenone, chloroform, and LiCl. Furthermore, it has been suggested that Betaine can suppress cancer cell growth in vivo and in vitro. Mitochondrial electron transport chain (ETC) complexes generate the mitochondrial membrane potential, which is essential to produce cellular energy, ATP. Reduced mitochondrial respiration and energy status have been found in many human pathological conditions including aging, cancer, and neurodegenerative disease.
METHODS AND RESULTS:
In this study we investigated whether Betaine directly targets mitochondria. We show that Betaine treatment leads to an upregulation of mitochondrial respiration and cytochrome c oxidase activity in H2.35 cells, the proposed rate limiting enzyme of ETC in vivo. Following treatment, the mitochondrial membrane potential was increased and cellular energy levels were elevated.
CONCLUSIONS:
We propose that the anti-proliferative effects of Betaine on cancer cells might be due to enhanced mitochondrial function contributing to a reversal of the Warburg effect.

Anti-inflammatory effects of betaine on AOM/DSS‑induced colon tumorigenesis in ICR male mice.[Pubmed: 24969167]

Int J Oncol. 2014 Sep;45(3):1250-6.

Betaine is an important human nutrient obtained from various foods and studies in animals and humans have provided results suggesting their pathogenesis of various chronic diseases and points to a role in risk assessment and disease prevention. However, the molecular mechanisms of its activity remain poorly understood and warrant further investigation.
METHODS AND RESULTS:
This study was performed to investigate the anti-inflammation and tumor preventing capacity of Betaine on colitis-associated cancer in mice. In in vivo experiments, we induced colon tumors in mice by azoxymethane (AOM) and dextran sulfate sodium (DSS) and evaluated the effects of Betaine on tumor growth. Administration with Betaine significantly decreased the incidence of tumor formation with downregulation of inflammation. Treatment with Betaine inhibited ROS generation and GSSG concentration in colonic mucosa. Based on the qPCR data, administration of Betaine inhibited inflammatory cytokines such TNF-α, IL-6, iNOS and COX-2. In in vitro experiments, LPS-induced NF-κB and inflammatory-related cytokines were inhibited by Betaine treatment in RAW 264.7 murine macrophage cells.
CONCLUSIONS:
Our findings suggest that Betaine is one of the candidates for the prevention of inflammation-associated colon carcinogenesis.

In vivo

Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease.[Pubmed: 24819676]

Br J Pharmacol. 2014 Sep;171(17):4073-86.

Overactive lipolysis in adipose tissue contributes to the pathogenesis of alcoholic liver disease (ALD); however, the mechanisms involved have not been elucidated. We previously reported that chronic alcohol consumption produces a hypomethylation state in adipose tissue. In this study we investigated the role of hypomethylation in adipose tissue in alcohol-induced lipolysis and whether its correction contributes to the well-established hepatoprotective effect of Betaine in ALD.
METHODS AND RESULTS:
Male C57BL/6 mice were divided into four groups and started on one of four treatments for 5 weeks: isocaloric pair-fed (PF), alcohol-fed (AF), PF supplemented with Betaine (BT/AF) and AF supplemented with Betaine (BT/AF). Betaine, 0.5% (w v(-1) ), was added to the liquid diet. Both primary adipocytes and mature 3T3-L1 adipocytes were exposed to demethylation reagents and their lipolytic responses determined. Betaine alleviated alcohol-induced pathological changes in the liver and rectified the impaired methylation status in adipose tissue, concomitant with attenuating lipolysis. In adipocytes, inducing hypomethylation activated lipolysis through a mechanism involving suppression of protein phosphatase 2A (PP2A), due to hypomethylation of its catalytic subunit, leading to increased activation of hormone-sensitive lipase (HSL). In line with in vitro observations, reduced PP2A catalytic subunit methylation and activity, and enhanced HSL activation, were observed in adipose tissue of alcohol-fed mice. Betaine attenuated this alcohol-induced PP2A suppression and HSL activation.
CONCLUSIONS:
In adipose tissue, a hypomethylation state contributes to its alcohol-induced dysfunction and an improvement in its function may contribute to the hepatoprotective effects of Betaine in ALD.

Sulfobetaine (dimethylsulfoniopropionate) and glycine betaine show incompatible involvement in crucial Ehrlich ascites carcinoma in mice.[Pubmed: 25750300]

Anticancer Res. 2015 Mar;35(3):1475-80.


METHODS AND RESULTS:
The role of methylation reactions in cancer was examined using the methylating agents, sulfoBetaine [dimethylsulfonioproponate (DMSP)], and glycine Betaine (GB), in murine crucial Ehrlich ascites carcinoma (EAC) for up to 10 days. DMSP administration in EAC-bearing mice mitigated EAC, while GB administration clearly promoted EAC. However, the immune cell profiles did not differ largely between animals receiving DMSP and those receiving GB. Moreover, DMSP and GB had merely any effects on proliferation of EAC cells in vitro. Injection of DMSP into normal mice interestingly led to macrophage accumulation in the peritoneal cavity in a dose-dependent manner at early rearing.
CONCLUSIONS:
These results indicate that GB promoted EAC by the methylation of cancer promotor gene, whereas DMSP ameliorated EAC by the accumulation of activated macrophages with a rapid response and long life span during cancer progression.

Betaine decreases hyperhomocysteinemia, endoplasmic reticulum stress, and liver injury in alcohol-fed mice.[Pubmed: 12730887]

Gastroenterology. 2003 May;124(5):1488-99.

Alcohol-induced hyperhomocysteinemia has been reported in rats and humans. Hyperhomocysteinemia has been associated with endoplasmic reticulum (ER) stress leading to the activation of ER-dependent apoptosis or up-regulation of lipid synthesis. This novel ER stress mechanism of alcoholic liver injury was studied in the model of intragastric alcohol-fed mice.
METHODS AND RESULTS:
Effects of alcohol on gene expression were analyzed using cDNA microarrays, RT-PCR, and Western blots over a period of 6 weeks. Liver injury was examined by histologic staining and TUNEL. We observed fatty liver, increased hepatic necroinflammation and apoptosis, and hyperhomocysteinemia. Of 1176 toxicology-related genes, glucose-regulated proteins (GRP-78 and -94), growth arrest/DNA damage-inducible protein 153 (CHOP/GADD153), and caspase-12 indicative of an ER stress response were among the alcohol-responsive genes. Sterol regulatory element binding protein (SREBP-1) and HMG-CoA reductase also were enhanced with alcohol administration. RT-PCR and selective Western blots confirmed the alcohol-induced expression of ER stress-related apoptosis and lipid synthesis genes. Addition of 0.5% and maximal 1.5% Betaine to the alcohol diet reduced the elevated level of plasma homocysteine by 54% and more than 80% accompanied by a decrease in hepatic lipids and ER stress response. Betaine did not attenuate the ethanol-induced increase in tumor necrosis factor alpha or CD14 mRNA.
CONCLUSIONS:
The results strongly suggest that alcohol may modulate both apoptotic and fat synthetic gene expression through homocysteine-induced ER stress in chronic alcoholic mouse liver and that correction of hyperhomocysteinemia by Betaine or other approaches may be useful to prevent alcoholic liver disease.

Protocol of Betaine

Cell Research

Betaine stabilizes cell volume and protects against apoptosis in human corneal epithelial cells under hyperosmotic stress.[Pubmed: 23246691 ]

Exp Eye Res. 2013 Mar;108:33-41.

Cell lines:Human corneal limbal epithelial (HCLE) cells
Concentrations: 5 or 10 mM
Incubation Time: 16 h
Method:
Human corneal limbal epithelial (HCLE) cells exposed to culture medium for 16 h at 300 mOsm (isotonic) or 500 mOsm (hyperosmotic) in the presence or absence of Betaine (5 or 10 mM) are evaluated for cell volume changes; cell viability; and apoptosis.

Animal Research

Effects of betaine on lipopolysaccharide-induced memory impairment in mice and the involvement of GABA transporter 2[Pubmed: 22053950]

J Neuroinflammation. 2011; 8: 153.

Animal Models: Male ddY strain mice
Formulation: 0.9% saline
Dosages:0.081, 0.163, or 0.326 mmol/kg
Administration: s.c.

Betaine Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Betaine Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of Betaine

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 8.5361 mL 42.6803 mL 85.3606 mL 170.7213 mL 213.4016 mL
5 mM 1.7072 mL 8.5361 mL 17.0721 mL 34.1443 mL 42.6803 mL
10 mM 0.8536 mL 4.268 mL 8.5361 mL 17.0721 mL 21.3402 mL
50 mM 0.1707 mL 0.8536 mL 1.7072 mL 3.4144 mL 4.268 mL
100 mM 0.0854 mL 0.4268 mL 0.8536 mL 1.7072 mL 2.134 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University
Featured Products
New Products
 

References on Betaine

Betaine decreases hyperhomocysteinemia, endoplasmic reticulum stress, and liver injury in alcohol-fed mice.[Pubmed:12730887]

Gastroenterology. 2003 May;124(5):1488-99.

BACKGROUND & AIMS: Alcohol-induced hyperhomocysteinemia has been reported in rats and humans. Hyperhomocysteinemia has been associated with endoplasmic reticulum (ER) stress leading to the activation of ER-dependent apoptosis or up-regulation of lipid synthesis. This novel ER stress mechanism of alcoholic liver injury was studied in the model of intragastric alcohol-fed mice. METHODS: Effects of alcohol on gene expression were analyzed using cDNA microarrays, RT-PCR, and Western blots over a period of 6 weeks. Liver injury was examined by histologic staining and TUNEL. RESULTS: We observed fatty liver, increased hepatic necroinflammation and apoptosis, and hyperhomocysteinemia. Of 1176 toxicology-related genes, glucose-regulated proteins (GRP-78 and -94), growth arrest/DNA damage-inducible protein 153 (CHOP/GADD153), and caspase-12 indicative of an ER stress response were among the alcohol-responsive genes. Sterol regulatory element binding protein (SREBP-1) and HMG-CoA reductase also were enhanced with alcohol administration. RT-PCR and selective Western blots confirmed the alcohol-induced expression of ER stress-related apoptosis and lipid synthesis genes. Addition of 0.5% and maximal 1.5% Betaine to the alcohol diet reduced the elevated level of plasma homocysteine by 54% and more than 80% accompanied by a decrease in hepatic lipids and ER stress response. Betaine did not attenuate the ethanol-induced increase in tumor necrosis factor alpha or CD14 mRNA. CONCLUSIONS: The results strongly suggest that alcohol may modulate both apoptotic and fat synthetic gene expression through homocysteine-induced ER stress in chronic alcoholic mouse liver and that correction of hyperhomocysteinemia by Betaine or other approaches may be useful to prevent alcoholic liver disease.

Anti-inflammatory effects of betaine on AOM/DSSinduced colon tumorigenesis in ICR male mice.[Pubmed:24969167]

Int J Oncol. 2014 Sep;45(3):1250-6.

Betaine is an important human nutrient obtained from various foods and studies in animals and humans have provided results suggesting their pathogenesis of various chronic diseases and points to a role in risk assessment and disease prevention. However, the molecular mechanisms of its activity remain poorly understood and warrant further investigation. This study was performed to investigate the anti-inflammation and tumor preventing capacity of Betaine on colitis-associated cancer in mice. In in vivo experiments, we induced colon tumors in mice by azoxymethane (AOM) and dextran sulfate sodium (DSS) and evaluated the effects of Betaine on tumor growth. Administration with Betaine significantly decreased the incidence of tumor formation with downregulation of inflammation. Treatment with Betaine inhibited ROS generation and GSSG concentration in colonic mucosa. Based on the qPCR data, administration of Betaine inhibited inflammatory cytokines such TNF-alpha, IL-6, iNOS and COX-2. In in vitro experiments, LPS-induced NF-kappaB and inflammatory-related cytokines were inhibited by Betaine treatment in RAW 264.7 murine macrophage cells. Our findings suggest that Betaine is one of the candidates for the prevention of inflammation-associated colon carcinogenesis.

Betaine is a positive regulator of mitochondrial respiration.[Pubmed:25498545]

Biochem Biophys Res Commun. 2015 Jan 9;456(2):621-5.

Betaine protects cells from environmental stress and serves as a methyl donor in several biochemical pathways. It reduces cardiovascular disease risk and protects liver cells from alcoholic liver damage and nonalcoholic steatohepatitis. Its pretreatment can rescue cells exposed to toxins such as rotenone, chloroform, and LiCl. Furthermore, it has been suggested that Betaine can suppress cancer cell growth in vivo and in vitro. Mitochondrial electron transport chain (ETC) complexes generate the mitochondrial membrane potential, which is essential to produce cellular energy, ATP. Reduced mitochondrial respiration and energy status have been found in many human pathological conditions including aging, cancer, and neurodegenerative disease. In this study we investigated whether Betaine directly targets mitochondria. We show that Betaine treatment leads to an upregulation of mitochondrial respiration and cytochrome c oxidase activity in H2.35 cells, the proposed rate limiting enzyme of ETC in vivo. Following treatment, the mitochondrial membrane potential was increased and cellular energy levels were elevated. We propose that the anti-proliferative effects of Betaine on cancer cells might be due to enhanced mitochondrial function contributing to a reversal of the Warburg effect.

Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease.[Pubmed:24819676]

Br J Pharmacol. 2014 Sep;171(17):4073-86.

BACKGROUND AND PURPOSE: Overactive lipolysis in adipose tissue contributes to the pathogenesis of alcoholic liver disease (ALD); however, the mechanisms involved have not been elucidated. We previously reported that chronic alcohol consumption produces a hypomethylation state in adipose tissue. In this study we investigated the role of hypomethylation in adipose tissue in alcohol-induced lipolysis and whether its correction contributes to the well-established hepatoprotective effect of Betaine in ALD. EXPERIMENTAL APPROACH: Male C57BL/6 mice were divided into four groups and started on one of four treatments for 5 weeks: isocaloric pair-fed (PF), alcohol-fed (AF), PF supplemented with Betaine (BT/AF) and AF supplemented with Betaine (BT/AF). Betaine, 0.5% (w v(-1) ), was added to the liquid diet. Both primary adipocytes and mature 3T3-L1 adipocytes were exposed to demethylation reagents and their lipolytic responses determined. KEY RESULTS: Betaine alleviated alcohol-induced pathological changes in the liver and rectified the impaired methylation status in adipose tissue, concomitant with attenuating lipolysis. In adipocytes, inducing hypomethylation activated lipolysis through a mechanism involving suppression of protein phosphatase 2A (PP2A), due to hypomethylation of its catalytic subunit, leading to increased activation of hormone-sensitive lipase (HSL). In line with in vitro observations, reduced PP2A catalytic subunit methylation and activity, and enhanced HSL activation, were observed in adipose tissue of alcohol-fed mice. Betaine attenuated this alcohol-induced PP2A suppression and HSL activation. CONCLUSIONS AND IMPLICATIONS: In adipose tissue, a hypomethylation state contributes to its alcohol-induced dysfunction and an improvement in its function may contribute to the hepatoprotective effects of Betaine in ALD.

Sulfobetaine (dimethylsulfoniopropionate) and glycine betaine show incompatible involvement in crucial Ehrlich ascites carcinoma in mice.[Pubmed:25750300]

Anticancer Res. 2015 Mar;35(3):1475-80.

BACKGROUND/AIM: The role of methylation reactions in cancer was examined using the methylating agents, sulfoBetaine [dimethylsulfonioproponate (DMSP)], and glycine Betaine (GB), in murine crucial Ehrlich ascites carcinoma (EAC) for up to 10 days. RESULTS: DMSP administration in EAC-bearing mice mitigated EAC, while GB administration clearly promoted EAC. However, the immune cell profiles did not differ largely between animals receiving DMSP and those receiving GB. Moreover, DMSP and GB had merely any effects on proliferation of EAC cells in vitro. Injection of DMSP into normal mice interestingly led to macrophage accumulation in the peritoneal cavity in a dose-dependent manner at early rearing. CONCLUSION: These results indicate that GB promoted EAC by the methylation of cancer promotor gene, whereas DMSP ameliorated EAC by the accumulation of activated macrophages with a rapid response and long life span during cancer progression.

Description

Betaine is used to treat homocystinuria.

Keywords:

Betaine,107-43-7,Natural Products, buy Betaine , Betaine supplier , purchase Betaine , Betaine cost , Betaine manufacturer , order Betaine , high purity Betaine

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: