DemecolcineCAS# 477-30-5 |
Quality Control & MSDS
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| Cas No. | 477-30-5 | SDF | Download SDF |
| PubChem ID | 220401 | Appearance | Powder |
| Formula | C21H25NO5 | M.Wt | 371.4 |
| Type of Compound | N/A | Storage | Desiccate at -20°C |
| Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
| Chemical Name | (7S)-1,2,3,10-tetramethoxy-7-(methylamino)-6,7-dihydro-5H-benzo[a]heptalen-9-one | ||
| SMILES | CNC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC | ||
| Standard InChIKey | NNJPGOLRFBJNIW-HNNXBMFYSA-N | ||
| Standard InChI | InChI=1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
Demecolcine Dilution Calculator
Demecolcine Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.6925 mL | 13.4626 mL | 26.9251 mL | 53.8503 mL | 67.3129 mL |
| 5 mM | 0.5385 mL | 2.6925 mL | 5.385 mL | 10.7701 mL | 13.4626 mL |
| 10 mM | 0.2693 mL | 1.3463 mL | 2.6925 mL | 5.385 mL | 6.7313 mL |
| 50 mM | 0.0539 mL | 0.2693 mL | 0.5385 mL | 1.077 mL | 1.3463 mL |
| 100 mM | 0.0269 mL | 0.1346 mL | 0.2693 mL | 0.5385 mL | 0.6731 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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Combined Treatment with Demecolcine and 6-Dimethylaminopurine during Postactivation Improves Developmental Competence of Somatic Cell Nuclear Transfer Embryos in Pigs.[Pubmed:28358237]
Anim Biotechnol. 2018 Jan 2;29(1):41-49.
This study determined the effects of postactivation treatment with Demecolcine and/or 6-dimethylaminopurine (6-DMAP) on in vivo and in vitro developmental competence of somatic cell nuclear transfer (SCNT) embryos in pigs. SCNT embryos were treated for 4 hours with 0.4 microg/mL Demecolcine, 2 mM 6-DMAP, or both after electric activation, then transferred to surrogate pigs or cultured for 7 days. The formation rate of SCNT embryos with a single pronucleus was higher in combined treatment with Demecolcine and 6-DMAP (95.2%) than treatment with Demecolcine alone (87.1%). Blastocyst formation of SCNT embryos was significantly increased in combined treatment with Demecolcine and 6-DMAP (48.7%) compared with Demecolcine (22.2%) or 6-DMAP alone (37.3%). Fluctuation of maturation promoting factor activity showed different patterns among various postactivation treatments. Pregnancy was established in 1 of 5 surrogates after transfer of SCNT embryos that were treated with Demecolcine and 6-DMAP. The pregnant surrogate delivered one healthy live piglet. The results of our study demonstrated that postactivation treatment with Demecolcine and 6-DMAP together improved preimplantation development and supported normal in vivo development of SCNT pig embryos, probably influencing MPF activity and nuclear remodeling, including induction of single pronucleus formation after electric activation.
