o-3M3FBSCAS# 313981-55-4 |
2D Structure
- Cefditoren Pivoxil
Catalog No.:BCC4898
CAS No.:117467-28-4
- Cefoselis
Catalog No.:BCC4092
CAS No.:122841-10-5
- Balofloxacin
Catalog No.:BCC4892
CAS No.:127294-70-6
- Pefloxacin Mesylate Dihydrate
Catalog No.:BCC5089
CAS No.:149676-40-4
- Tinidazole
Catalog No.:BCC4866
CAS No.:19387-91-8
- Toltrazuril
Catalog No.:BCC4870
CAS No.:69004-03-1
Quality Control & MSDS
3D structure
Package In Stock
Number of papers citing our products
Cas No. | 313981-55-4 | SDF | Download SDF |
PubChem ID | 770820 | Appearance | Powder |
Formula | C16H16F3NO2S | M.Wt | 343.36 |
Type of Compound | N/A | Storage | Desiccate at -20°C |
Solubility | Soluble to 100 mM in ethanol and to 100 mM in DMSO | ||
Chemical Name | 2,4,6-trimethyl-N-[2-(trifluoromethyl)phenyl]benzenesulfonamide | ||
SMILES | CC1=CC(=C(C(=C1)C)S(=O)(=O)NC2=CC=CC=C2C(F)(F)F)C | ||
Standard InChIKey | SKJJIFRWCCSXGL-UHFFFAOYSA-N | ||
Standard InChI | InChI=1S/C16H16F3NO2S/c1-10-8-11(2)15(12(3)9-10)23(21,22)20-14-7-5-4-6-13(14)16(17,18)19/h4-9,20H,1-3H3 | ||
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. |
Description | Inactive analog of m-3M3FBS. Used as a negative control. |
o-3M3FBS Dilution Calculator
o-3M3FBS Molarity Calculator
1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
1 mM | 2.9124 mL | 14.562 mL | 29.124 mL | 58.2479 mL | 72.8099 mL |
5 mM | 0.5825 mL | 2.9124 mL | 5.8248 mL | 11.6496 mL | 14.562 mL |
10 mM | 0.2912 mL | 1.4562 mL | 2.9124 mL | 5.8248 mL | 7.281 mL |
50 mM | 0.0582 mL | 0.2912 mL | 0.5825 mL | 1.165 mL | 1.4562 mL |
100 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.5825 mL | 0.7281 mL |
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. |
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
- FLI-06
Catalog No.:BCC5110
CAS No.:313967-18-9
- [Des-octanoyl]-Ghrelin (human)
Catalog No.:BCC7304
CAS No.:313951-59-6
- 13-Oxo-9,11-octadecadienoic acid
Catalog No.:BCC8437
CAS No.:31385-09-8
- PD 118057
Catalog No.:BCC7499
CAS No.:313674-97-4
- Bombesin
Catalog No.:BCC5708
CAS No.:31362-50-2
- INH1
Catalog No.:BCC6040
CAS No.:313553-47-8
- T0070907
Catalog No.:BCC2261
CAS No.:313516-66-4
- VU 590 dihydrochloride
Catalog No.:BCC7803
CAS No.:313505-85-0
- Reversan
Catalog No.:BCC7764
CAS No.:313397-13-6
- Regadenoson
Catalog No.:BCC6438
CAS No.:313348-27-5
- ICA 121431
Catalog No.:BCC6358
CAS No.:313254-51-2
- Aristolochic acid A
Catalog No.:BCN6262
CAS No.:313-67-7
- PU 02
Catalog No.:BCC6265
CAS No.:313984-77-9
- [cPP1-7,NPY19-23,Ala31,Aib32,Gln34] - hPancreatic Polypeptide
Catalog No.:BCC5750
CAS No.:313988-89-5
- VDM 11
Catalog No.:BCC7044
CAS No.:313998-81-1
- Evans Blue tetrasodium salt
Catalog No.:BCC6815
CAS No.:314-13-6
- (R)-(-)-Apomorphine hydrochloride
Catalog No.:BCC7250
CAS No.:314-19-2
- BPTES
Catalog No.:BCC6506
CAS No.:314045-39-1
- IU1
Catalog No.:BCC2086
CAS No.:314245-33-5
- Isotachioside
Catalog No.:BCN5230
CAS No.:31427-08-4
- Nocodazole
Catalog No.:BCC3826
CAS No.:31430-18-9
- 4-Amino-3-nitrobenzophenone
Catalog No.:BCC8682
CAS No.:31431-19-3
- Mebendazole
Catalog No.:BCC9016
CAS No.:31431-39-7
- 6-Methoxysalicylic Acid
Catalog No.:BCC8288
CAS No.:3147-64-6
Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in mouse olfactory neurons.[Pubmed:25374507]
Front Cell Neurosci. 2014 Oct 21;8:336.
Phospholipase C (PLC) and internal Ca(2+) stores are involved in a variety of cellular functions. However, our understanding of PLC in mammalian olfactory sensory neurons (OSNs) is generally limited to its controversial role in odor transduction. Here we employed single-cell Ca(2+) imaging and molecular approaches to investigate PLC-mediated Ca(2+) responses and its isozyme gene transcript expression. We found that the pan-PLC activator m-3M3FBS (25 muM) induces intracellular Ca(2+) increases in vast majority of isolated mouse OSNs tested. Both the response amplitude and percent responding cells depend on m-3M3FBS concentrations. In contrast, the inactive analog o-3M3FBS fails to induce Ca(2+) responses. The m-3M3FBS-induced Ca(2+) increase is blocked by the PLC inhibitor U73122, while its inactive analog U73433 has no effect. Removal of extracellular Ca(2+) does not change significantly the m-3M3FBS-induced Ca(2+) response amplitude. Additionally, in the absence of external Ca(2+), we found that a subset of OSNs respond to an odorant mixture with small Ca(2+) increases, which are significantly suppressed by U73122. Furthermore, using reverse transcription polymerase chain reaction and real-time quantitative polymerase chain reaction, we found that multiple PLC isozyme gene transcripts are expressed in olfactory turbinate tissue in various levels. Using RNA in situ hybridization analysis, we further show expression of beta4, gamma1, gamma2 gene transcripts in OSNs. Taken together, our results establish that PLC isozymes are potent enzymes for mobilizing intracellular Ca(2+) in mouse OSNs and provide molecular insight for PLC isozymes-mediated complex cell signaling and regulation in the peripheral olfactory epithelium.
Phospholipase C-independent effects of 3M3FBS in murine colon.[Pubmed:19931239]
Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94.
The muscarinic receptor subtype M(3) is coupled to Gq/11 proteins. Muscarinic receptor agonists such as carbachol stimulate these receptors that result in activation of phospholipase C (PLC) which hydrolyzes phosphatidylinositol 4,5-bisphosphate into diacylglycerol and Ins(1,4,5)P(3). This pathway leads to excitation and smooth muscle contraction. In this study the PLC agonist, 2, 4, 6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benezenesulfonamide (m-3M3FBS), was used to investigate whether direct PLC activation mimics carbachol-induced excitation. We examined the effects of m-3M3FBS and 2, 4, 6-trimethyl-N-(ortho-3-trifluoromethyl-phenyl)-benzenesulfonamide (o-3M3FBS), on murine colonic smooth muscle tissue and cells by performing conventional microelectrode recordings, isometric force measurements and patch clamp experiments. Application of m-3M3FBS decreased spontaneous contractility in murine colonic smooth muscle without affecting the resting membrane potential. Patch clamp studies revealed that delayed rectifier K(+) channels were reversibly inhibited by m-3M3FBS and o-3M3FBS. The PLC inhibitor, 1-(6-((17b-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione (U73122), did not prevent this inhibition by m-3M3FBS. Both m-3M3FBS and o-3M3FBS decreased two components of delayed rectifier K(+) currents in the presence of tetraethylammonium chloride or 4-aminopyridine. Ca(2+) currents were significantly suppressed by m-3M3FBS and o-3M3FBS with a simultaneous increase in intracellular Ca(2+). Pretreatment with U73122 did not prevent the decrease in Ca(2+) currents upon m-3M3FBS application. In conclusion, both m-3M3FBS and o-3M3FBS inhibit inward and outward currents via mechanisms independent of PLC acting in an antagonistic manner. In contrast, both compounds also caused an increase in [Ca(2+)](i) in an agonistic manner. Therefore caution must be employed when interpreting their effects at the tissue and cellular level.
Identification of a compound that directly stimulates phospholipase C activity.[Pubmed:12695532]
Mol Pharmacol. 2003 May;63(5):1043-50.
Phosphoinositide-specific phospholipase C (PLC) plays a pivotal role in the signal transduction of various cellular responses. However, although it is undeniably important that modulators of PLC activity be identified, no direct PLC activity modulator has been identified until now. In this study, by screening more than 10,000 different compounds in human neutrophils, we identified a compound that strongly enhances superoxide-generating activity, which is well known to be PLC-dependent. The active compound 2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide (m-3M3FBS) stimulated a transient intracellular calcium concentration ([Ca(2+)](i)) increase in neutrophils. Moreover, m-3M3FBS stimulated the formation of inositol phosphates in U937 cells, indicating that it stimulates PLC activity. The compound showed no cell-type specificity in terms of [Ca(2+)](i) increase in the various cell lines including leukocytes, fibroblasts, and neuronal cells. We also ruled out the possible involvement of heterotrimeric G proteins in m-3M3FBS-stimulated signaling by confirming the following: 1) pertussis toxin does not inhibit m-3M3FBS-induced [Ca(2+)](i) increase; 2) m-3M3FBS does not stimulate cyclic AMP generation; and 3) the inhibition of G(q) by the regulator of G protein-signaling 2 does not affect the m-3M3FBS-induced [Ca(2+)](i) increase. We also observed that m-3M3FBS stimulated PLC activity in vitro. The purified isoforms of PLC that were tested (i.e., beta2, beta3, gamma1, gamma2, and delta1) were activated by m-3M3FBS and showed no isoform specificity. Taken together, these results demonstrate that m-3M3FBS modulates neutrophil functions by directly activating PLC. Because m-3M3FBS is the first compound known to directly activate PLC, it should prove useful in the study of the basic molecular mechanisms of PLC activation and PLC-mediated cell signaling.