Home >> Research Area >>Others>>Others>> sodium 4-pentynoate

sodium 4-pentynoate

chemical reporter for the profiling of acetylated proteins CAS# 101917-30-0

sodium 4-pentynoate

Catalog No. BCC1958----Order now to get a substantial discount!

Product Name & Size Price Stock
sodium 4-pentynoate: 5mg $23 In Stock
sodium 4-pentynoate: 10mg Please Inquire In Stock
sodium 4-pentynoate: 20mg Please Inquire Please Inquire
sodium 4-pentynoate: 50mg Please Inquire Please Inquire
sodium 4-pentynoate: 100mg Please Inquire Please Inquire
sodium 4-pentynoate: 200mg Please Inquire Please Inquire
sodium 4-pentynoate: 500mg Please Inquire Please Inquire
sodium 4-pentynoate: 1000mg Please Inquire Please Inquire
Related Products

Quality Control of sodium 4-pentynoate

Number of papers citing our products

Chemical structure

sodium 4-pentynoate

3D structure

Chemical Properties of sodium 4-pentynoate

Cas No. 101917-30-0 SDF Download SDF
PubChem ID 66577022 Appearance Powder
Formula C5H5NaO2 M.Wt 120.08
Type of Compound N/A Storage Desiccate at -20°C
Solubility H2O : 100 mg/mL (832.78 mM; Need ultrasonic)
DMSO : 16.67 mg/mL (138.82 mM; Need ultrasonic)
Chemical Name sodium;pent-4-ynoate
SMILES C#CCCC(=O)[O-].[Na+]
Standard InChIKey IWIICCMJXWKGSG-UHFFFAOYSA-M
Standard InChI InChI=1S/C5H6O2.Na/c1-2-3-4-5(6)7;/h1H,3-4H2,(H,6,7);/q;+1/p-1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of sodium 4-pentynoate

Descriptionsodium 4-pentynoate is a alkynylacetate analogue; can be metabolically incorporated onto cellular proteins through biosynthetic mechanisms for profiling of acetylated proteins in diverse cell types.

References:
[1]. Yang YY, et al. Bioorthogonal chemical reporters for monitoring protein acetylation. J Am Chem Soc. 2010 Mar 24;132(11):3640-1.

sodium 4-pentynoate Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

sodium 4-pentynoate Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of sodium 4-pentynoate

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 8.3278 mL 41.6389 mL 83.2778 mL 166.5556 mL 208.1945 mL
5 mM 1.6656 mL 8.3278 mL 16.6556 mL 33.3111 mL 41.6389 mL
10 mM 0.8328 mL 4.1639 mL 8.3278 mL 16.6556 mL 20.8195 mL
50 mM 0.1666 mL 0.8328 mL 1.6656 mL 3.3311 mL 4.1639 mL
100 mM 0.0833 mL 0.4164 mL 0.8328 mL 1.6656 mL 2.0819 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University

Background on sodium 4-pentynoate

Sodium 4-pentynoate is an alkynylacetate analogue, which could be incorporated with cellular proteins and utilized as chemical reporter for the profiling of acetylated proteins [1].

Reversible protein acetylation is generally regulated by lysine acetyltransferases (KATs) and lysine deacetylases (KDACs), which play important roles in regulating gene expression and occurrence of disease [1].

The p300-KAT-catalyzed acetylation with histone protein H3 showed that the the 4-pentynoate group can be efficiently transferred onto lysine residue. In jurkat cells, metabolic labeling of sodium 4-pentynoate was in time and dose dependent, which was optimal at 2.5-10 μM of acetate analogs for 6-8 hr. Fluorescent profiling identified 194 of 4-pentynoate labeled proteins from jurkat cells, where 86% of them were identified by radioactive method and 14% was newly identified [1].

Reference:
[1].  Yang Y Y, et al. Bioorthogonal chemical reporters for monitoring protein acetylation. J Am Chem Soc., 2010, 132(11): 3640-3641.

Featured Products
New Products
 

References on sodium 4-pentynoate

Bioorthogonal chemical reporters for monitoring protein acetylation.[Pubmed:20192265]

J Am Chem Soc. 2010 Mar 24;132(11):3640-1.

Protein acetylation is a key post-translational modification that regulates diverse biological activities in eukaryotes. Here we report bioorthogonal chemical reporters that enable direct in-gel fluorescent visualization and proteome-wide identification of acetylated proteins via Cu(I)-catalyzed azide-alkyne cycloaddition, often termed "click chemistry". We demonstrate that two alkynyl-acetyl-CoA analogues, 4-pentynoyl-CoA and 5-hexynoyl-CoA, function as efficient substrates of lysine acetyltransferase p300 and serve as sensitive reagents for monitoring p300-catalyzed protein acetylation in vitro. In addition, we demonstrate that three alkynylacetate analogues, sodium 3-butynoate, sodium 4-pentynoate, and sodium 5-hexynoate, can be metabolically incorporated onto cellular proteins through biosynthetic mechanisms for profiling of acetylated proteins in diverse cell types. Mass spectrometry analysis of the enriched 4-pentynoate-labeled proteins revealed many reported as well as new candidate acetylated proteins from Jurkat T cells and specific sites of lysine acetylation. The bioorthogonal chemical reporters described here should serve as powerful tools for investigating protein acetylation.

Description

sodium 4-pentynoate is a alkynylacetate analogue; can be metabolically incorporated onto cellular proteins through biosynthetic mechanisms for profiling of acetylated proteins in diverse cell types.

Keywords:

sodium 4-pentynoate,101917-30-0,Natural Products,Others, buy sodium 4-pentynoate , sodium 4-pentynoate supplier , purchase sodium 4-pentynoate , sodium 4-pentynoate cost , sodium 4-pentynoate manufacturer , order sodium 4-pentynoate , high purity sodium 4-pentynoate

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: