WS 3

TRPM8 receptor agonist CAS# 1421227-52-2

WS 3

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Chemical structure

WS 3

3D structure

Chemical Properties of WS 3

Cas No. 1421227-52-2 SDF Download SDF
PubChem ID 72551586 Appearance Powder
Formula C28H30F3N7O3 M.Wt 569.58
Type of Compound N/A Storage Desiccate at -20°C
Solubility DMSO : ≥ 43 mg/mL (75.49 mM)
*"≥" means soluble, but saturation unknown.
Chemical Name N-[6-[4-[[4-[(4-methylpiperazin-1-yl)methyl]-3-(trifluoromethyl)phenyl]carbamoylamino]phenoxy]pyrimidin-4-yl]cyclopropanecarboxamide
SMILES CN1CCN(CC1)CC2=C(C=C(C=C2)NC(=O)NC3=CC=C(C=C3)OC4=NC=NC(=C4)NC(=O)C5CC5)C(F)(F)F
Standard InChIKey KIKOYRNAERIVSJ-UHFFFAOYSA-N
Standard InChI InChI=1S/C28H30F3N7O3/c1-37-10-12-38(13-11-37)16-19-4-5-21(14-23(19)28(29,30)31)35-27(40)34-20-6-8-22(9-7-20)41-25-15-24(32-17-33-25)36-26(39)18-2-3-18/h4-9,14-15,17-18H,2-3,10-13,16H2,1H3,(H2,34,35,40)(H,32,33,36,39)
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of WS 3

DescriptionWS3 is a novel small molecule that promotes β cell proliferation with EC50 of 28 nM(induced R7T1 proliferation). EC50 value: 28 nM [1] Target: β cell proliferation agonist Protocol(Only for Reference) [1] In vivo efficacy studies in RIP-DTA mice: RIP-DTA mice were purchased from Jackson Laboratories and all aspects of their treatment were outlined in a protocol approved by an internal animal care and use committee (IACUC). Mice were housed as 3-5 mice per cage and fed standard chow and water. During disease induction, doxycycline was added to the drinking water at a concentration of 2 mg/mL. Animals were injected intraperitoneally with saline-formulated WS6 at 5 mg/kg once every other day (for efficacy study). Blood glucose was monitored every third day. Mice were sacrificed at the end of week 1 and pancreas was fixed in formalin, sectioned, and stained for Ki67 and insulin. At the end of week 6, wet pancreas weight was recorded, and fixed pancreata were sectioned and stained for insulin; total insulin area and wet weight were used to calculate beta cell mass. For pharmacokinetic studies, WS6 was formulated at 50 mg/kg and given as single intraperitoneal dose, followed by serum collection and analysis of compound concentration in serum to determine CMAX and half-life (T1/2).

References:
[1]. Shen W, et al. Small-molecule inducer of β cell proliferation identified by high-throughput screening. J Am Chem Soc. 2013 Feb 6;135(5):1669-72.

WS 3 Dilution Calculator

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Preparing Stock Solutions of WS 3

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.7557 mL 8.7784 mL 17.5568 mL 35.1136 mL 43.892 mL
5 mM 0.3511 mL 1.7557 mL 3.5114 mL 7.0227 mL 8.7784 mL
10 mM 0.1756 mL 0.8778 mL 1.7557 mL 3.5114 mL 4.3892 mL
50 mM 0.0351 mL 0.1756 mL 0.3511 mL 0.7023 mL 0.8778 mL
100 mM 0.0176 mL 0.0878 mL 0.1756 mL 0.3511 mL 0.4389 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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Background on WS 3

WS 3 is an agonist of TRPM8 receptor with EC50 value of 3.7 μM [1].

Transient receptor potential cation channel subfamily M member 8 (TRPM8) is a Ca2+- and Na+-permeable ion channel and is activated by cooling agents and cold temperatures. TRPM8 belongs to TRP family and is expressed in sensory neurons.

WS 3 is an agonist of TRPM8 receptor and a cooling agent. In HEK293 cells expressed recombinant mouse TRPM8, WS 3 (30 μM) increased [Ca2+]i. WS 3 exhibited potency with EC50 value of 3.7 μM and induced 86% efficacy of the maximal response to icilin, the most potent agonist [1]. In HEK cells stably expressing either hTRPM8 or hTRPA1, WS 3 activated hTRPM8 and hTRPA1 with EC50 values of 2.2 and 120.6 μM, respectively. WS 3 was efficacious in eliciting cooling sensation [2].

References:
[1].  Behrendt HJ, Germann T, Gillen C, et al. Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay. Br J Pharmacol, 2004, 141(4): 737-745.
[2].  Klein AH, Iodi Carstens M, McCluskey TS, et al. Novel menthol-derived cooling compounds activate primary and second-order trigeminal sensory neurons and modulate lingual thermosensitivity. Chem Senses, 2011, 36(7): 649-658.

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References on WS 3

Abnormal photocurrent response and enhanced photocatalytic activity induced by charge transfer between WS(2) nanosheets and WO(3) nanoparticles.[Pubmed:24227745]

Chemphyschem. 2013 Dec 16;14(18):4069-73.

Sun trap: Pure WS2 nanosheets are prepared that exhibit excellent photosensitive properties. After functionalization with WO3 nanoparticles, abnormal photocurrent responses, enhanced photocatalytic activity, and induced photoluminescence is observed.

WS-50030 [7-{4-[3-(1H-inden-3-yl)propyl]piperazin-1-yl}-1,3-benzoxazol-2(3H)-one]: a novel dopamine D2 receptor partial agonist/serotonin reuptake inhibitor with preclinical antipsychotic-like and antidepressant-like activity.[Pubmed:19828876]

J Pharmacol Exp Ther. 2010 Jan;332(1):190-201.

The preclinical characterization of WS-50030 [7-{4-[3-(1H-inden-3-yl)propyl]piperazin-1-yl}-1,3-benzoxazol-2(3H)-one] is described. In vitro binding and functional studies revealed highest affinity to the D(2) receptor (D(2L) K(i), 4.0 nM) and serotonin transporter (K(i), 7.1 nM), potent D(2) partial agonist activity (EC(50), 0.38 nM; E(max), 30%), and complete block of the serotonin transporter (IC(50), 56.4 nM). Consistent with this in vitro profile, WS-50030 (10 mg/kg/day, 21 days) significantly increased extracellular 5-HT in the rat medial prefrontal cortex, short-term WS-50030 treatment blocked apomorphine-induced climbing (ID(50), 0.51 mg/kg) in a dose range that produced minimal catalepsy in mice and induced low levels of contralateral rotation in rats with unilateral substantia nigra 6-hydroxydopamine lesions (10 mg/kg i.p.), a behavioral profile similar to that of the D(2) partial agonist aripiprazole. In a rat model predictive of antipsychotic-like activity, WS-50030 and aripiprazole reduced conditioned avoidance responding by 42 and 55% at 10 mg/kg, respectively. Despite aripiprazole's reported lack of effect on serotonin transporters, long-term treatment with aripiprazole or WS-50030 reversed olfactory bulbectomy-induced hyperactivity at doses that did not reduce activity in sham-operated rats, indicating antidepressant-like activity for both compounds. Despite possessing serotonin reuptake inhibitory activity in addition to D(2) receptor partial agonism, WS-50030 displays activity in preclinical models predictive of antipsychotic- and antidepressant efficacy similar to aripiprazole, suggesting potential efficacy of WS-50030 versus positive and negative symptoms of schizophrenia, comorbid mood symptoms, bipolar disorder, major depressive disorder, and treatment-resistant depression. Furthermore, WS-50030 provides a tool to further explore how combining these mechanisms might differentiate from other antipsychotics or antidepressants.

Stepwise addition of CuNCS onto [Et(4)N][Tp*WS(3)]: design, syntheses, structures and third-order nonlinear optical properties.[Pubmed:19381405]

Dalton Trans. 2009 May 14;(18):3425-33.

Stepwise reactions of [Et(4)N][Tp*WS(3)] () (Tp* = hydridotris(3,5-dimethylpyrazol-1-yl)borate) with 1-4 equiv. of CuNCS (and Et(4)NBr in the case of three equiv. of CuNCS) afforded the [1 + 1] to [1 + 4] addition products [Et(4)N][Tp*WS(mu-S)(2)(CuNCS)].0.5CH(2)Cl(2) (.0.5CH(2)Cl(2)), [Et(4)N][Tp*W(mu(3)-S)(mu-S)(2)(CuNCS)(2)].ClCH(2)CH(2)Cl (.ClCH(2)CH(2)Cl), [Et(4)N](2)[Tp*W(mu(3)-S)(3)(CuNCS)(3)(mu(3)-Br)].1.5aniline (.1.5aniline), and {[Et(4)N][Tp*W(mu(3)-S)(3)(Cu-mu-SCN)(3)(Cu-mu(3)-NCS)]}(n) (). Compounds were characterized by elemental analysis, IR spectra, UV-vis spectra, (1)H NMR, and single-crystal X-ray crystallography. The cluster anion of contains a [WS(2)Cu] core formed by addition of one CuNCS group onto the [Tp*WS(3)] species. The cluster anion of has a butterfly-shaped [WS(3)Cu(2)] core constructed by addition of two CuNCS groups onto the [Tp*WS(3)] species. The cluster dianion of consists of a cubane-like [Tp*WS(3)Cu(3)(mu(3)-Br)] core assembled by addition of three CuNCS groups onto the [Tp*WS(3)] species followed by filling a mu(3)-Br into the void of the incomplete cubane-like [Tp*WS(3)(CuNCS)(3)] fragment. has a 2D cluster-supported layer network in which each [Tp*WS(3)Cu(3)] core acting as a pyramidal 3-connecting node interconnects with the [Cu(NCS)(4)] units through thiocyanate bridges. In addition, the third-order nonlinear optical (NLO) performances of in DMF were also investigated by Z-scan techniques.

Description

β cell proliferation agonist WS3 is a novel small molecule that promotes β cell proliferation with EC50 of 28 nM(induced R7T1 proliferation).

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