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CB-839

CAS# 1439399-58-2

CB-839

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Chemical structure

CB-839

3D structure

Chemical Properties of CB-839

Cas No. 1439399-58-2 SDF Download SDF
PubChem ID 71577426 Appearance Powder
Formula C26H24F3N7O3S M.Wt 571.57
Type of Compound N/A Storage Desiccate at -20°C
Solubility DMSO : ≥ 30 mg/mL (52.49 mM)
H2O : < 0.1 mg/mL (insoluble)
*"≥" means soluble, but saturation unknown.
Chemical Name N-[6-[4-[5-[(2-pyridin-2-ylacetyl)amino]-1,3,4-thiadiazol-2-yl]butyl]pyridazin-3-yl]-2-[3-(trifluoromethoxy)phenyl]acetamide
SMILES C1=CC=NC(=C1)CC(=O)NC2=NN=C(S2)CCCCC3=NN=C(C=C3)NC(=O)CC4=CC(=CC=C4)OC(F)(F)F
Standard InChIKey PRAAPINBUWJLGA-UHFFFAOYSA-N
Standard InChI InChI=1S/C26H24F3N7O3S/c27-26(28,29)39-20-9-5-6-17(14-20)15-22(37)31-21-12-11-18(33-34-21)7-1-2-10-24-35-36-25(40-24)32-23(38)16-19-8-3-4-13-30-19/h3-6,8-9,11-14H,1-2,7,10,15-16H2,(H,31,34,37)(H,32,36,38)
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Protocol

Cell Assay [1]
For viability assays, all cell lines are treated with CB-839 at the indicated concentrations for 72 hours in duplicate wells and analyzed for antiproliferative effects using Cell Titer Glo. For all cell lines except MDA-MB-175, SUM149PT, Hs343.T, HCC38, and BT20 the results present represent an average across at least two independent experiments. IC50 values are calculated using a four parameter curve fit. Relative cell loss or proliferation in the presence of 1 μM CB-839 or in glutamine-free media is determined by comparing the CTG signals.

Animal Administration [1]
Tumor growth studies are done in two xenograft models: (i) a patient-derived TNBC model, where tumor fragments isolated from the breast tissue of a 53-year-old Caucasian woman with stage IIa infiltrating ductal carcinoma are implanted subcutaneously into female nu/nu mice (age 4-6 weeks, 19-26 g) and (ii) a cell line model, where JIMT-1 cells are implanted subcutaneously at 1×107cells per mouse in the flank of female CB.17 SCID mice (age 8-12 weeks, 17-23 g). In both models, when tumors reach approximately 100-150 mm3, mice are dosed with vehicle or 200 mg/kg CB-839 (n=10/group) prepared in vehicle orally twice daily every 12 hours for 28 to 35 days. For the JIMT-1 model, two additional cohorts are dosed with paclitaxel prepared in 5% ethanol/5% Cremophor EL given as an intravenous bolus at 10 mg/kg every other day for 5 doses alone or in combination with 200 mg/kg CB-839 dosed orally twice daily. Tumor volumes and body weights are measured twice weekly.

References:
[1]. Gross MI, et al. Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer. Mol Cancer Ther. 2014 Apr;13(4):890-901. [2]. Reisz JA, et al. Red blood cells in hemorrhagic shock: a critical role for glutaminolysis in fueling alanine transamination in rats. Blood Adv. 2017 Jul 14;1(17):1296-1305. [3]. Gregory MA, et al. Glutaminase inhibition improves FLT3 inhibitor therapy for acute myeloid leukemia. Exp Hematol. 2018 Feb;58:52-58.

CB-839 Dilution Calculator

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Preparing Stock Solutions of CB-839

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.7496 mL 8.7478 mL 17.4957 mL 34.9913 mL 43.7392 mL
5 mM 0.3499 mL 1.7496 mL 3.4991 mL 6.9983 mL 8.7478 mL
10 mM 0.175 mL 0.8748 mL 1.7496 mL 3.4991 mL 4.3739 mL
50 mM 0.035 mL 0.175 mL 0.3499 mL 0.6998 mL 0.8748 mL
100 mM 0.0175 mL 0.0875 mL 0.175 mL 0.3499 mL 0.4374 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

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Background on CB-839

CB-839 is a potent and orally bioavailable inhibitor of both splice variants of glutaminase (KGA and GAC), and with IC50s of 23 nM and 28 nM in kidney and brain, respectively.

In Vitro:CB-839 has increased potency and distinct kinetic behavior, exhibiting a slow-on/slow-off mechanism. CB-839 has a potent effect on the proliferation of HCC1806 and MDA-MB-231 cell lines (IC50 of 20-55 nM associated with cell loss at >100 nM). TNBC cell lines are sensitive to glutaminase inhibition with CB-839[1].

In Vivo:CB-839 (200 mg/kg, p.o.) has antitumor activity in xenograft models of TNBC and basal-like breast cancer, and inhibits tumor glutaminase activity and changes metabolite levels[1].

References:
[1]. Gross MI, et al. Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer. Mol Cancer Ther. 2014 Apr;13(4):890-901. [2]. Reisz JA, et al. Red blood cells in hemorrhagic shock: a critical role for glutaminolysis in fueling alanine transamination in rats. Blood Adv. 2017 Jul 14;1(17):1296-1305. [3]. Gregory MA, et al. Glutaminase inhibition improves FLT3 inhibitor therapy for acute myeloid leukemia. Exp Hematol. 2018 Feb;58:52-58.

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References on CB-839

Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer.[Pubmed:24523301]

Mol Cancer Ther. 2014 Apr;13(4):890-901.

Glutamine serves as an important source of energy and building blocks for many tumor cells. The first step in glutamine utilization is its conversion to glutamate by the mitochondrial enzyme glutaminase. CB-839 is a potent, selective, and orally bioavailable inhibitor of both splice variants of glutaminase (KGA and GAC). CB-839 had antiproliferative activity in a triple-negative breast cancer (TNBC) cell line, HCC-1806, that was associated with a marked decrease in glutamine consumption, glutamate production, oxygen consumption, and the steady-state levels of glutathione and several tricarboxylic acid cycle intermediates. In contrast, no antiproliferative activity was observed in an estrogen receptor-positive cell line, T47D, and only modest effects on glutamine consumption and downstream metabolites were observed. Across a panel of breast cancer cell lines, GAC protein expression and glutaminase activity were elevated in the majority of TNBC cell lines relative to receptor positive cells. Furthermore, the TNBC subtype displayed the greatest sensitivity to CB-839 treatment and this sensitivity was correlated with (i) dependence on extracellular glutamine for growth, (ii) intracellular glutamate and glutamine levels, and (iii) GAC (but not KGA) expression, a potential biomarker for sensitivity. CB-839 displayed significant antitumor activity in two xenograft models: as a single agent in a patient-derived TNBC model and in a basal like HER2(+) cell line model, JIMT-1, both as a single agent and in combination with paclitaxel. Together, these data provide a strong rationale for the clinical investigation of CB-839 as a targeted therapeutic in patients with TNBC and other glutamine-dependent tumors.

Description

Telaglenastat (CB-839) is a first-in-class, selective, reversible and orally active glutaminase 1 (GLS1) inhibitor. Telaglenastat selectively inhibits GLS1 splice variants KGA (kidney-type glutaminase) and GAC (glutaminase C) compared to GLS2. The IC50s are 23 nM and 28 nM for endogenous glutaminase in mouse kidney and brain, respectively. Telaglenastat inudces autophagy and has antitumor activity.

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