Home >> Research Area >>Apoptosis>>Caspase>> Q-VD-OPh hydrate

Q-VD-OPh hydrate

Cell-permeable, irreversible pan-caspase inhibitor CAS# 1135695-98-5

Q-VD-OPh hydrate

2D Structure

Catalog No. BCC1125----Order now to get a substantial discount!

Product Name & Size Price Stock
Q-VD-OPh hydrate: 5mg $322 In Stock
Q-VD-OPh hydrate: 10mg Please Inquire In Stock
Q-VD-OPh hydrate: 20mg Please Inquire Please Inquire
Q-VD-OPh hydrate: 50mg Please Inquire Please Inquire
Q-VD-OPh hydrate: 100mg Please Inquire Please Inquire
Q-VD-OPh hydrate: 200mg Please Inquire Please Inquire
Q-VD-OPh hydrate: 500mg Please Inquire Please Inquire
Q-VD-OPh hydrate: 1000mg Please Inquire Please Inquire
Related Products

Quality Control of Q-VD-OPh hydrate

3D structure

Package In Stock

Q-VD-OPh hydrate

Number of papers citing our products

Chemical Properties of Q-VD-OPh hydrate

Cas No. 1135695-98-5 SDF Download SDF
PubChem ID 24794416 Appearance Powder
Formula C26H25F2N3O6 M.Wt 513.49
Type of Compound N/A Storage Desiccate at -20°C
Synonyms QVD-OPH; Quinoline-Val-Asp-Difluorophenoxymethylketone
Solubility DMSO : 93.33 mg/mL (181.76 mM; Need ultrasonic)
Chemical Name (3S)-5-(2,6-difluorophenoxy)-3-[[(2S)-3-methyl-2-(quinoline-2-carbonylamino)butanoyl]amino]-4-oxopentanoic acid
SMILES CC(C)C(C(=O)NC(CC(=O)O)C(=O)COC1=C(C=CC=C1F)F)NC(=O)C2=NC3=CC=CC=C3C=C2
Standard InChIKey OOBJCYKITXPCNS-REWPJTCUSA-N
Standard InChI InChI=1S/C26H25F2N3O6/c1-14(2)23(31-25(35)19-11-10-15-6-3-4-9-18(15)29-19)26(36)30-20(12-22(33)34)21(32)13-37-24-16(27)7-5-8-17(24)28/h3-11,14,20,23H,12-13H2,1-2H3,(H,30,36)(H,31,35)(H,33,34)/t20-,23-/m0/s1
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Biological Activity of Q-VD-OPh hydrate

DescriptionQ-VD-OPH is a selective, brain and cell permeable, highly potent and irreversible inhibitor of caspase-3 ( IC50=25nm), caspase-1 (IC50=50nM), caspase-8 (IC50=100nM) and caspase-9 (IC50=430nM).
Targetscaspase-1caspase-3caspase-8caspase-9   
IC5050nM25nm100nM430nM   

Protocol

Cell experiment[1]:

Cell lines

JURL-MK1 and HL60 cell

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Reacting condition

No specific suggestion

Applications

Q-VD-OPh largely inhibited caspase-3 and 7 activity at 0.05 mM. Caspase-8 was also inhibited by Q-VD-OPh at very low concentration. Q-VD-OPh prevented the cleavage of PARP-1 at 10 mM . Q-VD-OPh inhibited DNA fragmentation and disruption of the cell membrane functionality at 2 mM, and the drug-induced loss of cellular adhesivity to fibronectin need 10 mM Q-VD-OPh.

Animal experiment [2]:

Animal models

TgCRND8 mice in 3 months-old

Dosage form

Intraperitoneally Injected with 10 mg/kg QVD-OPh at 3 times a week for 3 months

Application

Q-VD-OPh inhibited caspase-7 activation and limited the pathological changes of tau and caspase cleavage in chronic treatment of Alzheimer disease.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

1. Kuželová K1, Grebeňová D, Brodská B.Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes. J Cell Biochem. 2011 Nov;112(11):3334-42.

2. Rohn TT, Kokoulina P, Eaton CR et al. Caspase activation in transgenic mice with Alzheimer-like pathology: results from a pilot study utilizing the caspase inhibitor, Q-VD-OPh.  Int J Clin Exp Med. 2009 Nov 5;2(4):300-8.

Q-VD-OPh hydrate Dilution Calculator

Concentration (start)
x
Volume (start)
=
Concentration (final)
x
Volume (final)
 
 
 
C1
V1
C2
V2

calculate

Q-VD-OPh hydrate Molarity Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
g/mol

calculate

Preparing Stock Solutions of Q-VD-OPh hydrate

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.9475 mL 9.7373 mL 19.4746 mL 38.9492 mL 48.6864 mL
5 mM 0.3895 mL 1.9475 mL 3.8949 mL 7.7898 mL 9.7373 mL
10 mM 0.1947 mL 0.9737 mL 1.9475 mL 3.8949 mL 4.8686 mL
50 mM 0.0389 mL 0.1947 mL 0.3895 mL 0.779 mL 0.9737 mL
100 mM 0.0195 mL 0.0974 mL 0.1947 mL 0.3895 mL 0.4869 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Organizitions Citing Our Products recently

 
 
 

Calcutta University

University of Minnesota

University of Maryland School of Medicine

University of Illinois at Chicago

The Ohio State University

University of Zurich

Harvard University

Colorado State University

Auburn University

Yale University

Worcester Polytechnic Institute

Washington State University

Stanford University

University of Leipzig

Universidade da Beira Interior

The Institute of Cancer Research

Heidelberg University

University of Amsterdam

University of Auckland
TsingHua University
TsingHua University
The University of Michigan
The University of Michigan
Miami University
Miami University
DRURY University
DRURY University
Jilin University
Jilin University
Fudan University
Fudan University
Wuhan University
Wuhan University
Sun Yat-sen University
Sun Yat-sen University
Universite de Paris
Universite de Paris
Deemed University
Deemed University
Auckland University
Auckland University
The University of Tokyo
The University of Tokyo
Korea University
Korea University

Background on Q-VD-OPh hydrate

The broad spectrum caspase inhibitor, QVD-OPh, provides a cost effective, non toxic, and highly specific means of apoptotic inhibition and provides new insight into the design of new inhibitors1.

Actinomycin D rapidly induced apoptosis and this was dramatically inhibited by the caspase inhibitor, Q-VD-OPh (quinolyl-valyl-O-methylaspartyl-[-2, 6-difluorophenoxy]-methyl ketone). Q-VD-OPh was significantly more effective in preventing apoptosis than the widely used inhibitors, ZVAD-fmk and Boc-D-fmk. Q-VD-OPh was also equally effective in preventing apoptosis mediated by the three major apoptotic pathways, caspase 9/3, caspase 8/10, and caspase 12. In addition to the increased effectiveness, Q-VD-OPh was not toxic to cells, even at high concentrations.

Q-VD-OPh was equally effective at inhibiting the three major apoptotic pathways, was functional in different cell types and species (human, mouse, and rat) and prevented terminal caspase activation, substrate cleavage, and DNA ladder formation associated with apoptosis. Q-VD-OPh can inhibit recombinant caspases 1, 3, 8, and 9 with IC50 values ranging from 25 to 400 nM2. The effectiveness of Q-VD-OPh as an apoptotic inhibitor is evidenced by the complete suppression of an apoptotic inducer capable of inducing substantial cell death in less than 4 hours.

Q-VD-OPh protected against the substantial apoptosis induced by actinomycin D. In addition, Q-VD-OPh alone exhibited little or no toxicity, even at extremely high concentrations.

The effective concentration of Q-VD-OPh may provide a unique reagent when trying to revive hard to propagate cell lines from liquid nitrogen. The addition of this inhibitor to thawed cells would give the cells adequate time to recover, even in the presence of standard DMSO concentrations (10%), from the stress of thawing and begin to proliferate in the absence of toxicity. Q-VD-OPh is stable in solution for several months and is effective in culture for at least 2.5 days. This would provide an ideal time frame for cell recovery; whereas, the subsequent decrease in effectiveness over time would be fortuitous in that the cells would return to standard culture conditions with minimal manipulation1.

References:
1. T. M. Caserta, A. N. Smith, A. D. Gultice, M. A. Reedy and T. L. Brown, Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties, Apoptosis 2003; 8: 345–352
2. Yin XM. Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res 2000; 10: 161–167

Featured Products
New Products
 

Description

Q-VD-OPh is an irreversible pan-caspase inhibitor with potent antiapoptotic properties; inhibits caspase 7 with an IC50 of 48 nM and 25-400 nM for other caspases including caspase 1, 3, 8, 9, 10, and 12. Q-VD-OPh can inhibits HIV infection. Q-VD-OPh is able to cross the blood-brain barrier.

Keywords:

Q-VD-OPh hydrate,1135695-98-5,QVD-OPH; Quinoline-Val-Asp-Difluorophenoxymethylketone,Natural Products,Caspase, buy Q-VD-OPh hydrate , Q-VD-OPh hydrate supplier , purchase Q-VD-OPh hydrate , Q-VD-OPh hydrate cost , Q-VD-OPh hydrate manufacturer , order Q-VD-OPh hydrate , high purity Q-VD-OPh hydrate

Online Inquiry for:

      Fill out the information below

      • Size:Qty: - +

      * Required Fields

                                      Result: